罗氏DNA双杂交探针

罗氏DNA双杂交探针

易于使用的快速高性能 qPCR 试剂盒，适用于所有基于LightCycler ® 转盘的仪器的HybProbe 信号传输。

For general laboratory use.

供一般实验室使用。

Pack Size：96 x 20 µL reactions  包装尺寸：96 x 20 µL 反应

Pack Size：480 x 20 µL reactions   包装尺寸：480 x 20 µL 反应

Overview概述：

Easy-to-use reaction mix for PCR, using HybProbe probes with the LightCycler® Carousel-Based System.

易于使用的 PCR 反应混合物，使用 HybProbe 探针和 LightCycler ® 基于旋转木马的系统。

LightCycler® DNA Master HybProbe provides robust performance and convenience for standard PCR applications.

LightCycler ®  DNA Master HybProbe 为标准 PCR 应用提供强大的性能和便利性。

Contents内容

LightCycler® DNA Master HybProbes

双杂交探针® DNA主要杂交探针

MgCl2 Stock Solution, (25 mM)

MgCl 2 储备溶液，（25 mM）

Water, PCR-grade

水，PCR 级

Application应用：

LightCycler® DNA Master HybProbe is an easy-to-use reaction mix for sensitive PCR applications in LightCycler® capillaries, using HybProbe probes as detection format. The kit can be used to perform quantitative PCR, as well as SNP and mutation detection. The addition of MgCl2 (provided in the kit) for specific applications may be necessary.

LightCycler ®  DNA Master HybProbe 是一种易于使用的反应混合物，适用于 LightCycler ® 毛细管中的灵敏 PCR 应用，使用 HybProbe 探针作为检测形式。该试剂盒可用于进行定量 PCR，以及 SNP 和突变检测。 可能需要为特定应用添加 MgCl 2（在套件中提供）。

Detail细节：

The unique LightCycler® HybProbe format is based on the principle of fluorescence resonance energy transfer (FRET). Two sequence-specific oligonucleotide probes, labeled with different dyes (donor and acceptor), are added to the reaction mix in addition to PCR primers. HybProbe probes hybridize to the target sequences on the amplified DNA fragment during the annealing phase in a head-to-tail arrangement, thereby bringing the two dyes into close proximity. The donor dye (fluorescein) is excited by the blue light LED source and the energy emitted by the donor dye excites the acceptor dye attached to the second HybProbe probe, which then emits fluorescent light at a different wavelength. The amount of fluorescence is directly proportional to the amount of target DNA generated during the PCR process.

Note:  All LightCycler® Master Mixes have been specifically developed to function in LightCycler® Capillaries and provide optimal performance and sensitivity.

*的 LightCycler ®  HybProbe 格式基于荧光共振能量转移 (FRET) 原理。除了 PCR 引物外，还向反应混合物中加入了两种序列特异性寡核苷酸探针，用不同染料（供体和受体）标记。HybProbe 探针在退火阶段以头对尾的方式与扩增的 DNA 片段上的目标序列杂交，从而使两种染料接近。供体染料（荧光素）被蓝光 LED 光源激发，供体染料发射的能量激发附着在第二个 HybProbe 探针上的受体染料，然后发射不同波长的荧光。荧光量与 PCR 过程中产生的目标 DNA 量成正比。

注意：  所有 LightCycler® 预混液均经过专门开发，可在 LightCycler® 毛细管中发挥作用并提供最佳性能和灵敏度。

Quality 质量

The LightCycler® DNA Master HybProbe is function tested using the LightCycler® Control Kit DNA, according to the kit protocols.

根据试剂盒协议，LightCycler ®  DNA Master HybProbe 使用 LightCycler ® Control Kit DNA进行功能测试。