Illumina TruSeq Stranded Total RNA Kit

Illumina TruSeq Stranded Total RNA Kit

Illumina/TruSeq Stranded Total RNA Kit with Ribo-Zero Plant, Set B (48 samples, 12 indexes)/RS-122-2402/1 Ea

Illumina

产品编号： RS-122-2402

美  元  价： $5630.00

品       牌： Illumina

产       地： 美国

Library Preparation Kits "Product Highlights:

High-Quality Whole-Transcriptome Analysis with Precise Strand Information

RNA-Seq technology provides uniform coverage, precise measurement of strand orientation and high-confidence discovery of features such as alternative transcripts, antisense expression and allele-specific expression across both coding and multiple forms of noncoding RNA. The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs

The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. The kit has been validated for use in multiple plant species, including ArABIdopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:

Assay Time ~6.5 hours

Hands-On Time ~3.5 hours

Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)

Mechanism of Action Probe hybridization, extension-ligation, and PCR

Multiplexing 1–96

Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb

Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)

System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000

Species Category Other,Bovine,Mouse,Human,Rat

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Automation CapABIlity Liquid Handling Robots

Method Amplicon Sequencing,Custom Sequencing

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