花青-3- dUTP [Cy 3-dUTP] :DNA标记与检测的高效工具
时间:2024-12-30 阅读:33
染料修饰的脱氧尿苷5'-三磷酸盐(如氨基烯丙基dUTP)可用于通过常规酶掺入方法(如逆转录、缺口翻译、随机引物标记或PCR)生产含染料的DNA。这种酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。这种Cy3-dUTP缀合物可以用Spectrum Orange™滤光片组作为红色荧光色(Spectrum Oranges™是Vysis的商标)。它在功能上与Cyanine 3-dUTP(Perkin-Elmer的NEL578001EA)相当。
艾美捷花青-3- dUTP [Cy 3-dUTP] :
货号 AAT-17025
单位大小:25纳摩尔
分子量:1150.00
溶剂:水
预期用途 仅供研究使用(RUO)
储存条件:冷冻(<-15°C);尽量减少光照
校正因子(260纳米):0.07
校正因子(280纳米):0.073
消光系数(cm -1 M -1):150000
激发(纳米):555
发射(纳米):569
量子产率:0.15
图:Cy3和iFluor®555 dUTP标记的端粒探针在中期HeLa细胞中的荧光原位杂交。
花青-3- dUTP [Cy 3-dUTP] 文献参考:
A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375
Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77
Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52
Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9
Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6