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花青-3- dUTP [Cy 3-dUTP] :DNA标记与检测的高效工具

时间:2024-12-30      阅读:33

染料修饰的脱氧尿苷5'-三磷酸盐(如氨基烯丙基dUTP)可用于通过常规酶掺入方法(如逆转录、缺口翻译、随机引物标记或PCR)生产含染料的DNA。这种酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。这种Cy3-dUTP缀合物可以用Spectrum Orange™滤光片组作为红色荧光色(Spectrum Oranges™是Vysis的商标)。它在功能上与Cyanine 3-dUTPPerkin-ElmerNEL578001EA)相当。

 

艾美捷花青-3- dUTP [Cy 3-dUTP] 

货号 AAT-17025

单位大小:25纳摩尔

分子量:1150.00

溶剂:水

预期用途 仅供研究使用(RUO

储存条件:冷冻(<-15°C);尽量减少光照

校正因子(260纳米):0.07

校正因子(280纳米):0.073

消光系数(cm -1 M -1):150000

激发(纳米):555

发射(纳米):569

量子产率:0.15

31.png

图:Cy3iFluor®555 dUTP标记的端粒探针在中期HeLa细胞中的荧光原位杂交。

 

花青-3- dUTP [Cy 3-dUTP] 文献参考:

A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.

Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia

Journal: Journal of agricultural and food chemistry (2020): 369-375

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.

Authors: Gutjahr, Alice and Xu, Shuang-yong

Journal: Nucleic acids research (2014): e77

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.

Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua

Journal: Analytical chemistry (2012): 5645-52

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.

Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki

Journal: The Analyst (2011): 2252-9

Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.

Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan

Journal: Animal reproduction science (2011): 32-6


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