BiomomentumMach-1应用改变细胞外基质力学
时间:2021-03-18 阅读:409
Biomomentum多轴机械测试仪Mach-1应用:通过改变细胞外基质力学来控制肺血管通透性和内毒素诱导的肺水肿
Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics
- PMID: 23612300
- DOI: 10.1038/ncomms2774
Abstract
Increased vascular permeability contributes to many diseases, including acute respiratory distress syndrome, cancer and inflammation. Most past work on vascular barrier function has focused on soluble regulators, such as tumour-necrosis factor-α. Here we show that lung vascular permeability is controlled mechanically by changes in extracellular matrix structure. Our studies reveal that pulmonary vascular leakage can be increased by altering extracellular matrix compliance in vitro and by manipulating lysyl oxidase-mediated collagen crosslinking in vivo. Either decreasing or increasing extracellular matrix stiffness relative to normal levels disrupts junctional integrity and increases vascular leakage. Importantly, endotoxin-induced increases of vascular permeability are accompanied by concomitant increases in extracellular matrix rigidity and lysyl oxidase activity, which can be prevented by inhibiting lysyl oxidase activity. The identification of lysyl oxidase and the extracellular matrix as critical regulators of lung vascular leakage might lead to the development of new therapeutic approaches for the treatment of pulmonary oedema and other diseases caused by abnormal vascular permeability.
血管通透性增加导致许多疾病,包括急性呼吸窘迫综合征,癌症和炎症。过去有关血管屏障功能的大多数工作都集中在可溶性调节剂上,例如肿瘤坏死因子-α。在这里,我们显示肺血管通透性是由细胞外基质结构的变化机械地控制的。我们的研究表明,通过改变体外细胞外基质的顺应性和在体内操纵赖氨酰氧化酶介导的胶原蛋白交联可以增加肺血管渗漏。相对于正常水平降低或增加细胞外基质硬度都会破坏连接完整性并增加血管渗漏。重要的,内毒素诱导的血管通透性增加伴随着细胞外基质刚性和赖氨酰氧化酶活性的同时增加,这可以通过抑制赖氨酰氧化酶活性来预防。赖氨酰氧化酶和细胞外基质作为肺血管渗漏的关键调节剂的鉴定可能会导致开发新的治疗方法,用于治疗由异常的血管通透性引起的肺水肿和其他疾病。
biomomentum多轴机械测试仪Mach-1
Mach-1多轴机械测试仪是模块化集成压缩,拉伸,剪切,摩擦,扭转和3D压痕映射、电位分布等测试设备
biomomentum至1999年以来,专注用于测试生物材料,组织和关节软骨机-电特性产品的设计、开发、制造和商业化的创新解决方案20余年。
其mach-1多轴向多功能组织材料机械特性测试分析系统已经成为组织材料机械-电位测试分析的黄金标准。
1、多功能、多轴向,适用样品范围广:
•1.1、从骨等硬组织材料到脑组织、眼角膜等极软的组织材料
•1.2、从粗的椎间盘的样品到极细的单纤维丝
2、力学类型测试分析功能齐全:
2.1、模块化集成压缩、张力、剪切、摩擦、扭转、穿刺、摩擦和非平面压痕、3D厚度、3D表面轮廓等各种力学类型支持,微观结构表征及动态力学分析研究
2.2、多物理场耦合加载测试
•3、通高量压痕、压缩测试分析(48孔板中压痕测试分析)
•4、高精度、高分辨率:
•4.1、位移分辨率达0.1um
•4.2、力分辨率达0.025mN
•4.3、样品直径小25um
•5、行程范围广:50-250mm
•6、体积小巧、可放入培养箱内
•7 、DIC (Digital Image Correlation)数字图像相关法非接触式的高精度位移、应变测量
•9、活性组织电位分布测试分析
•10、产品成熟,文献量达上千篇