Carbapenem-resistant Klebsiella pneumoniae (CRKP)感染小鼠模型，全肺脏流式检测分群圈门策略，可以参考下图。Gating strategy for determining the myeloid and lymphoid subsets in the whole lungs of control and Trpv1DTA mice after 48h of clodronate liposome (CLL) administration. PBS-liposome (PBL) was used for control group of mice. CLL or PBL (70 μL/mouse) was inoculated via intratracheal route at 48 hours before the immune cell type analyses. 单核巨噬清除订购信息见后。

(A) Gating strategy for determining the myeloid and lymphoid subsets in the whole lungs of control and Trpv1DTA mice after 48h of clodronate liposome (CLL) administration. PBS-liposome (PBL) was used for control group of mice. CLL or PBL (70 μL/mouse) was inoculated via intratracheal route at 48 hours before the immune cell type analyses. Interstitial macrophages (IMs, CD45+Siglec-F–CD11b+Ly6G– F4/80+ cells), neutrophils (CD45+Siglec-F–CD11b+Ly6G+ cells), Ly6Chi monocytes (CD45+Siglec-F–CD11b+Ly6G– F4/80– Ly6Chi cells), Ly6Clo monocytes (CD45+Siglec-F–CD11b+Ly6G–F4/80–Ly6Clo cells), CD11b+ DC (CD45+Siglec-F–CD11b+Ly6G– F4/80–Ly6C– CD11c+ MHC-II+cells), CD11b– DCs (CD45+Siglec-F–CD11b– CD3– B220– NK1.1– CD11c+ MHC-II+cells), B cells (CD45+Siglec-F–CD11b– CD3–NK1.1–B220+cells), CD4+ T cells (CD45+Siglec-F–CD11b– CD3+ CD8–CD4+ cells), CD8+ T cells (CD45+Siglec-F–CD11b– CD3+ CD4–CD8+ cells) and CD4– CD8– T cells (CD45+Siglec-F–CD11b– CD3+ CD4–CD8– cells).

(B) Absolute numbers of myeloid and lymphoid subsets in the whole lungs of CLL- or PBL-treated control (n=3-4) and Trpv1DTA (n=3) mice after 48h of treatment.

(C) The BALF number of Ly6Chi monocytes, neutrophils, and AMs in CLL- and PBL-treated control (n=4) and Trpv1DTA mice (n=3-4) after 24h of CRKP infection. Statistical analysis: Mann Whitney test (B, left), unpaired ttests (B, right and C to E) with the following significance levels: (*P<0.05, **P<0.01,***P<0.001). ns, not significant.

品牌：Liposoma

产地：荷兰

货号：CP-005-005

规格：5ml+5ml

品名：Clodronateliposomes & Control Liposomes

代理：靶点科技

部位：肺脏

细胞：肺泡巨噬细胞（AM）

剂量：70ul

方式：滴鼻（intratracheal administration）

时间点：建模前48h

题目：Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection

期刊：SCIENCE ADVANCES

时间：6 Sep 2024

期卷：Vol 10, Issue 36

链接：DOI: 10.1126/sciadv.adl6162

Liposoma巨噬细胞清除剂氯膦酸二钠脂质体清除肺泡巨噬细胞AM：