Carbapenem-resistant Klebsiella pneumoniae (CRKP)感染小鼠模型，气管肺泡灌洗液 ( bronchoalveolar lavage fluid, BALF)流式检测分群圈门策略，可以参考下图。单核巨噬清除订购信息见后。

Gating strategy to characterize immune cells recovered in BALF. BALF cells were gated for live cells by excluding LIVE/DEAD fixable aqua-stained cells.

Leukocytes were gated as CD45+cells, which were further separated into Siglec-F+, CD11b+ and CD11b– cells. Siglec-F+ (SF+) cells were further gated into F4/80+ and CD11c+; both F4/80+ and CD11c+ cells were identified as alveolar macrophages (AMs). From CD11b+ cells, Ly6G+ cells were gated as neutrophils. CD11b+ Ly6G– cells were gated for interstitial macrophages (IMs) (CD11b+ F4/80+). Remaining cell populations were separated into Ly6Chi monocytes (CD11b+ Ly6Chi) and Ly6Clo monocytes (CD11b+ Ly6Clo). CD11b– cells were gated into B cells (B220+ cells) and remaining CD11b– B220–cells were separated into CD4+ T cells, CD8+ T cells, CD4+ CD8+ T cells and CD4– CD8– T cells(non-T & -B cells).

品牌：Liposoma

产地：荷兰

货号：CP-005-005

规格：5ml+5ml

品名：Clodronateliposomes & Control Liposomes

代理：靶点科技

部位：肺脏

细胞：肺泡巨噬细胞（AM）

剂量：70ul

方式：滴鼻（intratracheal administration）

时间点：建模前48h

题目：Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection

期刊：SCIENCE ADVANCES

时间：6 Sep 2024

期卷：Vol 10, Issue 36

链接：DOI: 10.1126/sciadv.adl6162

Liposoma巨噬细胞清除剂氯膦酸二钠脂质体清除肺泡巨噬细胞AM：