中文摘要：

与肌肉减少症相关的肌肉无力是导致老年人健康寿命和生活质量下降的主要原因。然而，衰老中肌肉无力的潜在机制尚未明确。我们研究了氧化应激和衰老对缺乏抗氧化酶 CuZnSod （Sod1KO） 的小鼠和衰老 （24 个月大） 野生型小鼠和皮肤透化单纤维产生肌肉力的特定分子机制的影响。两种模型都发生肌肉力量损失，可能是因为膜兴奋性降低，NKA 信号传导和 RyR 稳定性改变，纤维 Ca2+ 敏感性降低，并通过修饰 Cys674 残基抑制 SERCA 活性，SR 失调和胞质 Ca2+ 稳态，以及线粒体 Ca2+ 缓冲和呼吸受损。我们的结果提供了对衰老和氧化应激对肌肉无力相关机制的具体影响的更好理解，这可能为未来对抗肌肉无力的干预措施指明方向。

英文摘要：

Muscle weakness associated with sarcopenia is a major contributor to reduced health span and quality of life in the elderly. However, the underlying mechanisms of muscle weakness in aging are not fully defined. We investigated the effect of oxidative stress and aging on specific molecular mechanisms involved in muscle force production in mice and skinned permeabilized single fibers in mice lacking the antioxidant enzyme CuZnSod (Sod1KO) and in aging (24-month-old) wild-type mice. Loss of muscle strength occurs in both models, potentially because of reduced membrane excitability with altered NKA signaling and RyR stability, decreased fiber Ca2+ sensitivity and suppressed SERCA activity via modification of the Cys674 residue, dysregulated SR and cytosolic Ca2+ homeostasis, and impaired mitochondrial Ca2+ buffering and respiration. Our results provide a better understanding of the specific impacts of aging and oxidative stress on mechanisms related to muscle weakness that may point to future interventions for countering muscle weakness.

论文信息：

论文题目：Impact of aging and oxidative stress on specific components of excitation contraction coupling in regulating force generation

期刊名称：SCIENCE ADVANCES

时间期卷： Vol 8, Issue 43

在线时间：2022年10月26日

DOI： 10.1126/sciadv.add7377

Virogen抗谷胱甘肽抗体货号101-A检测图：

Virogen抗体101-A引用截图：

Virogen抗谷胱甘肽Anti-Glutathione antibody抗体 抗谷胱甘肽小鼠单克隆抗体 Anti-Glutathione monoclonal antibody 产品编号:101-A。该抗体检测谷胱甘肽 谷胱甘肽是啥: 谷胱甘肽是一种普遍存在于动物、植物和部分细菌体内的抗氧化剂,能够清除活性氧自由基,阻止脂质过氧化。