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ATAT1, GST-tag Recombinant
面议ATF2, GST-Tag Recombinant
面议GCN5 (727-837), His-tag Recombinant
面议GCN5 (KAT2A), FLAG-tag Recombinant
面议p300 (KAT3B), His-GST-tag Recombinant
面议ACHE, His-Tag Recombinant
面议APAF1, His-tag, FLAG-tag Recombinant
面议BCL-2, His-Tag Recombinant
面议BCL2A1, His-Tag Recombinant
面议BCL2L10, His-Tag Recombinant
面议Caspase6, His-tag Recombinant
面议Caspase7, His-tag Recombinant
面议The 3CL Protease (T21I, E166V) (SARS-CoV-2) Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the activity of T21I, E166V mutated 3CL Protease for screening and profiling applications, with no time-consuming washing steps. The kit contains enough purified 3CL Protease (T21I, E166V) (BPS Bioscience #101671), fluorogenic substrate, and 3CL Protease assay buffer for 100 enzyme reactions. 3CL inhibitor GC376 is also included as a control.
Figure 1: Illustration of the principle behind the 3CL protease assay.The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between the glutamine and serine residues to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience #79952).