Anti- Klotho抗体说明书，一抗

Mouse Anti- Klotho抗体说明书，一抗，单抗
FROM: ABCAM
Cat. Number: Rs-2925R
Quantity size: 0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）

Background: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D （By similarity）. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Specificity: •anti-Klotho is a mouse monoclonal antibody unconjugated
•specific for Klotho of human
•epitope mapping near the amino terminus ofKlotho of
human
• use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
• Protein A affinity chromatography purification, purity :>95%
• Isotype: IgG
• mol wt: 82kDa
Mouse Anti- Klotho抗体说明书，一抗，单抗

Application: • Western blotting: 1:100-500
• Immunohistochemistry: 1:100-500
• ELISA: 1:500-1000
• Optimal working dilutions must be determined by the end user.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Mouse Anti- Klotho抗体说明书，一抗，单抗