- 面议
参考价：
起订量：
Anti-Histone H3 antibody - ChIP Grade
- 型号
该企业相似产品
抗体
fantibody抗体搜索引擎是一个供公共检索的抗体数据库，其抗体信息数据来源于范围的研究机构与商业公司。该引擎由商品化抗体数据库与抗体应用评价数据库两部分组成，以帮助研究者更高效的寻找并评估该抗体的性能。抗体搜索引擎是继基因与蛋白数据库之后更为复杂的应用型检索平台，由探生科技建立并维护。
详细信息
基本信息
| 抗体名称 | Anti-Histone H3 antibody - ChIP Grade |
|---|---|
| 抗体简介 | Goat Polyclonal antibody |
| Goat polyclonal to Histone H3 - ChIP Grade | |
| 应用类型 | ChIP,IP,WB |
| 反应种属 | Mouse,Chicken,Dog,Human,Saccharomycescerevisiae,Xenopuslaevis,Fruitfly（Drosophilamelanogaster）,ChineseHamster |
| 免疫原 | Synthetic peptide derived from within residues 100 to the C-terminus of Human Histone H3. |
抗体属性
| 亚型 | IgG |
|---|---|
| 保存形态 | liquid |
| 抗体浓度 | 0.78 mg/ml |
| 缓冲体系 | Preservative: 0.02% Sodium Azide Constituents: PBS, pH 7.4 |
| 储存条件 | Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| 纯化 | Purified |
基因信息
| 基因名 | Histone H3 |
|---|---|
| 抗原名 | Histone H3 |
| 抗原别名 | H3.3B,H3F3A,H3histone,H3histonefamilymemberEpseudogene,H3histonefamilymemberEpseudogene,H3.3A,H33_HUMAN,H3F3,H3F3,H3f3b,HIST3H3,HIST3H3,HistoneH33pseudogene,HistoneH33pseudogene,HistoneH3.3,PP781, |
| 修饰说明 | Acetylation is generally linked to gene activation. Acetylation on Lys-10 （H3K9ac） impairs methylation at Arg-9 （H3R8me2s）. Acetylation on Lys-19 （H3K18ac） and Lys-24 （H3K24ac） favors methylation at Arg-18 （H3R17me）.Citrullination at Arg-9 （H3R8ci） and/or Arg-18 （H3R17ci） by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 （H3R17me2a） by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 （H3R8me2s） by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 （H3R2me2a） by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation （H3K4me2 and H3K4me3）. H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 （H3K4me）, Lys-37 and Lys-80. Methylation at Lys-5 （H3K4me） facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 （H3K79me） is associated with DNA double-strand break （DSB） responses and is a specific target for TP53BP1. Methylation at Lys-10 （H3K9me） and Lys-28 （H3K27me）, which are linked to gene repression, are underrepresented. Methylation at Lys-10 （H3K9me） is a specific target for HP1 proteins （CBX1, CBX3 and CBX5） and prevents subsequent phosphorylation at Ser-11 （H3S10ph） and acetylation of H3 and H4. Methylation at Lys-5 （H3K4me） and Lys-80 （H3K79me） require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 （H3K9me） and Lys-28 （H3K27me） are enriched in inactive X chromosome chromatin.Phosphorylated at Thr-4 （H3T3ph） by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 （H3S10ph） by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 （H3S10ph） by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 （H3S10ph）, which is linked to gene activation, prevents methylation at Lys-10 （H3K9me） but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 （H3S10ph） by AURKB mediates the dissociation of HP1 proteins （CBX1, CBX3 and CBX5） from heterochromatin. Phosphorylation at Ser-11 （H3S10ph） is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 （H3S28ph） by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 （H3T6ph） by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 （H3K4me） by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 （H3T11ph） from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 （H3T11ph） by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 （H3K9me） by KDM4C/JMJD2C. Phosphorylation at Tyr-42 （H3Y41ph） by JAK2 promotes exclusion of CBX5 （HP1 alpha） from chromatin. Phosphorylation on Ser-32 （H3S31ph） is specific to regions bordering centromeres in metaphase chromosomes.Ubiquitinated. Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V（D）J recombination. |
| 抗原家族 | Belongs to the histone H3 family. |
| 抗原功能 | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| 细胞定位 | Nucleus. Chromosome. |
| 分子量 | 15 kDa |
