- 面议
起订量：
80601 Configuration-specific Monoclonal Antibody BasedRhoA Activation Assay Kit
- 型号
- 80601
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武汉纽斯特生物技术有限公司是一家集生物医药产品的研发、生产、销售及技术服务为一体的中外合资企业，公司位于武汉东湖*开发区光谷生物城创新基地, 研发实验室占地面积1500平方米。公司研发团队30余人，其中博士4人，硕士10余人。公司自2006年创办以来，一直致力于生物医药领域高技术含量的产品的研发和销售，并始终站在生物医药领域的Z前沿，以细胞信号转导相关的创新药物筛选试剂盒, 癌症诊断试剂盒, 临床前评价药物对重要靶器官毒性的生物标志物的检测试剂盒以及个性化指导用药检测试剂盒为主要研发方向。公司现已建立起稳定完善的研发技术平台，研发出几十种具有*性的，拥有*自主知识产权的新药筛选试剂盒和癌症诊断试剂盒，并且迅速占领销售市场。公司已建立起活性蛋白质表达和纯化技术平台、结构特异性单克隆抗体和多克隆抗体研发技术平台、癌症分子诊断试剂盒研发技术平台等，具备完善的生产研发和质量控制体系，同时也初步建立起国内外销售网络，并与国内外许多大型的制药企业以及各重点高校科研院所建立了业务联系，逐步建立并形成了产——研——销相结合良性可持续发展的新型经济模式。
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详细信息
Configuration-specific Monoclonal Antibody Based
RhoA Activation Assay Kit
Catalog Number： 80601
20 assays
Product Description
Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family
of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the
activity of RhoA, and the hydrolysis of GTP to GDP renders it inactive.
Currently the activation of RhoA proteins is assayed with the binding of GTP-bound RhoA to the
RhoA-binding domain （RBD） of the RhoA effector protein, Rhotekin. This method is based on the
observation that the active, GTP-bound RhoA could bind to the RBD of rhotekin. However, the
reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to
GDP during the assay procedure, and the low binding affinity of RBD to RhoA-GTP.
NewEast Biosciences RhoA Activation Assay Kit is based on the configuration-specific monoclonal
antibody that specifically recognizes RhoA-GTP, but not RhoA-GDP. Given the high affinity of
monoclonal antibodies to their antigens, the activation assay could be performed in a much shorter time.
This assay provides the reliable results with consistent reproducibility.
This anti-RhoA-GTP monoclonal antibody can also be used to monitor the activation of RhoA in cells
and in tissues by immunohistochemistry.
NewEast Biosciences RhoA Activation Assay Kit provides a simple and fast method to monitor the
activation of RhoA. Each kit provides sufficient quantities to perform 20 assays.
Assay Principle
NewEast Biosciences RhoA Activation Assay Kit bases on the configuration-specific anti-RhoA-GTP
monoclonal antibody to measure the active RhoA-GTP levels, either from cell extracts or from in vitro
GTPγS loading RhoA activation assays. Briefly, anti-active RhoA mouse monoclonal antibody will be
incubated with cell lysates containing RhoA-GTP. The bound active RhoA will then be pulled down by
protein A/G agarose. The precipitated active RhoA will be detected by immunoblot analysis using
anti-RhoA rabbit polyclonal antibody.
