Configuration-specific Monoclonal Antibody Based
Arf6 Activation Assay Kit
Catalog Number： 82401
20 assays

Product Description
ARF6 （ADP-ribosylation factor 6） is a member of the ARF super-family. ARF genes encode small
GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular
trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a
regulatory molecule of phagocytosis.
Currently there is no direct assay to measure the activation of Arf 6 GTPase.
NewEast Biosciences Arf6 Activation Assay Kit is based on the configuration-specific monoclonal
antibody that specifically recognizes Arf6-GTP, but not Arf6-GDP, and an Arf6 specific rabbit
polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation
assay could be performed in a short time. This assay provides the reliable results with consistent
reproducibility.
These anti-Arf 6-GTP monoclonal antibodies can also be used to monitor the activation of Arf 6 in cells
and in tissues by immunohistochemistry.
NewEast Biosciences Arf6 Activation Assay Kit provides a simple and fast method to monitor the
activation of Arf 6. Each kit provides sufficient quantities to perform 20 assays.
Assay Principle
NewEast Biosciences Arf 6 Activation Assay Kit bases on the configuration-specific anti-Arf 6-GTP
monoclonal antibody to measure the active Arf 6-GTP levels, either from cell extracts or from in vitro
GTPγS loading Arf 6 activation assays. Briefly, anti-active Arf 6 mouse monoclonal antibody will be
incubated with cell lysates containing Arf 6-GTP. The bound active Arf 6 will then be pulled down by
protein A/G agarose. The precipitated active Arf 6 will be detected by immunoblot analysis using an
anti-Arf 6 rabbit polyclonal antibody, respectively.