公司拥有ABGENT、B-bridge、IBL-America、R&D、CST、MBL、SAB、KPL、Santa、Abnova、LifeSpan等磷酸化粘着斑激酶抗体品牌全年代理权，价格行业优势明显。
英文名称 Anti-Phospho-FAK/PTK2（Tyr397）
中文名称 磷酸化粘着斑激酶抗体
 磷酸化粘着斑激酶抗体别 名 FAK （phospho Y397）; p-FAK （phospho Y397）; PTK2（phospho Y397）; FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; Focal adhesion kinase 1; FRNK; pp125FAK; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; PTK 2; FAK1_HUMAN; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 转录调节因子 激酶和磷酸酶
321171,7-二羟基-3,4-二甲氧基山酮-7-O-葡萄糖苷1,7-Dihydroxy-3,4-dimethoxylxanthone-7-O-glucosideC21H22O11450.410mg
321183,3'-O-二甲基鞣花酸3,3’-Di-O-methylellagic acid "2239-88-5
""C16H10O8
"330.2510mg
32119鞣花酸Ellagic acid476-66-4C14H6O8302.1920mg
32120５,７-二羟基色原酮5,7-dihydroxychromone31721-94-5"C9H6O4
""178.15
"10mg
32121石蒜碱Lycorine 476-28-8C16H17NO4287.3120mg
32122盐酸石蒜碱Lycorine hydrochloride 2188-68-3 C16H17NO4287.31 20mg
32123氧化石蒜碱Lycobetaine72510-04-4C16H12NO3+266.2720mg
32124番茄红素Lycopene502-65-8C40H56536.8720mg
32125番泻苷ASennoside A81-27-6C42H38O20862.7420mg
32126番泻苷BSennoside B128-57-4C42H38O20862.7420mg
32127番泻苷CSennoside C37271-16-2C42H40O19848.7610mg
32128番泻苷DSennoside D37271-17-3C42H40O19848.75910mg
32129茴香脑cis-Anethol104-46-1C10H12O148.220mg
32130反式茴香脑cis-Anethol104-46-1C10H12O148.220mg
32131茯苓酸Pachymic acid29070-92-6C33H52O5528.7620mg
321323-氢化去氢松苓酸Dehydrotrametenolic acid4545mg
32133粉防己碱Tetrandrine518-34-3C38H42N2O6622.7520mg
32134防己诺林碱Fangchinoline436-77-1C37H40N2O6608.7220mg
32135佛手柑内酯Bergapten484-20-8C12H8O4216.1920mg
32136 异佛手柑内酯Isobergapten482-48-4C12H8O4216.18952 20mg
32137扶桑甾醇beta-rosasterol10mg
32138芳樟醇Linalool78-70-6C10H18O154.2520mg
32139附子灵FuzilineC24H39NO7 20mg
32140覆盆子酮4-（4-Hydroxyphenyl）-2-butanone5471-51-2C10H12O2164.220mg
32141风信子素2-（1-Ethoxyethoxy）ethyl）benzene2556-10-7C12H18O2194.2720mg
32142法卡林二醇Falcarindiol55297-87-5C17H24O2260.3710mg
32143羌活醇Notopterol88206-46-6C21H22O5354.420mg
32144肥皂草素（UV98%）Saponin8047-15-2C27H42O320mg
32145甘草酸Glycyrrhizic acid 1405-86-3C42H62O16822.9320mg
32146甘草酸单铵盐Glycyrrhizic acid ammonium salt53956-04-0C42H65NO16839.9620mg
32147甘草酸二铵Diammonium glycyrrhizinate79165-06-3C42H62O16.2H3N856.99720mg
32148甘草苷Liquiritin551-15-5C21H22O9418.39620mg
32149异甘草苷 Isoliquiritin 5041-81-6 C21H22O9418.39 20mg
32150甘草次酸18 β-Glycyrrhetintic Acid471-53-4C30H46O4470.6820mg
32151光甘草定Glabridin59870-68-7C20H20O4324.3720mg
32152甘草查尔酮ALicochalcone A58749-22-7C21H22O4338.420mg
32153甘草查尔酮BLicochalcone B58749-23-8C16H14O520mg
32154甘草查尔酮CLicochalcone C144506-14-9C21H22O420mg
32155甘草素Liquiritigenin578-86-9C15H12O4256.2520mg
32156异甘草素Isoliquiritigenin961-29-5C15H12O4256.2520mg
32157水甘草碱Tabersonine4429-63-4C21H24N2O2336.4320mg
磷酸化粘着斑激酶抗体32158鬼臼毒素Podophyllotoxin518-28-5C22H22O8414.4120mg
32159鬼臼毒素-4-O-葡萄糖苷Podophyllotoxin-4-O-glucoside 16481-54-2C28H32O1310mg
蛋白分子量 predicted molecular weight: 116kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH cunjugated Synthesised phosphopeptide derived from human FAK 1 around the phosphorylation site of Tyr397
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化粘着斑激酶抗体产品介绍 Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor （GPCR） occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Function : Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor （GPCR） occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Subunit : Interacts （via first Pro-rich region） with CAS family members （via SH3 domain）, including BCAR1, BCAR3, CASS4 and NEDD9. Interacts with GIT1. Interacts with SORBS1. Interacts with RGNEF. Interacts with SHB. Interacts with PXN and TLN1. Interacts with STAT1. Interacts with DCC. Interacts with WASL. Interacts with ARHGEF7. Interacts with GRB2 and GRB7 （By similarity）. Component of a complex that contains at least FER, CTTN and PTK2/FAK1. Interacts with BMX. Interacts with TGFB1I1. Interacts with STEAP4. Interacts with ZFYVE21. Interacts with ESR1. Interacts with PIK3R1 or PIK3R2. Interacts with SRC, FGR, FLT4 and RET. Interacts with EPHA2 in resting cells; activation of EPHA2 recruits PTPN11, leading to dephosphorylation of PTK2/FAK1 and dissociation of the complex. Interacts with EPHA1 （kinase activity-dependent）. Interacts with CD4; this interaction requires the presence of HIV-1 gp120. Interacts with PIAS1. Interacts with ARHGAP26 and SHC1. Interacts with RB1CC1; this inhibits PTK2/FAK1 activity and activation of downstream signaling pathways. Interacts with P53/TP53 and MDM2. Interacts with LPXN （via LD motif 3）.
Subcellular Location : Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Note=Constituent of focal adhesions. Detected at microtubules.
Tissue Specificity : Detected in B and T lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts （at protein level）. Ubiquitous.
Post-translational modifications : Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. FGR promotes phosphorylation at Tyr-397 and Tyr-576. FER promotes phosphorylation at Tyr-577, Tyr-861 and Tyr-925, even when cells are not adherent. Tyr-397, Tyr-576 and Ser-722 are phosphorylated only when cells are adherent. Phosphorylation at Tyr-397 is important for interaction with BMX, PIK3R1 and SHC1. Phosphorylation at Tyr-925 is important for interaction with GRB2. Dephosphorylated by PTPN11; PTPN11 is recruited to PTK2 via EPHA2 （tyrosine phosphorylated）. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly; this dephosphorylation could be catalyzed by PTPN11 and regulated by ZFYVE21.
Sumoylated; this enhances autophosphorylation.
DISEASE : Note=Aberrant PTK2/FAK1 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. PTK2/FAK1 overexpression is seen in many types of cancer.
Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family.
FAK subfamily.
Contains 1 FERM domain.
Database links : UniProtKB/Swiss-Prot: Q05397.2
FAK是整合蛋白介导的信号转导中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化,FAK本身是胱冬肽酶（caspase）的底物。作为信号分子的FAK参与抑制细胞凋亡并直接参与细胞多种功能的调节。
1.FAK 局部粘着斑激酶，是一种酪氨酸激酶;肿瘤细胞的侵袭性生长是一个多步骤的复杂过程，有多种生物化学因子参与其中,局部粘着斑激酶（focal adhesion kinase, FAK）介导的信号转导系统就是其中zui为重要的细胞信号转导途径之一。肿瘤细胞必须黏附于细胞外基质，通过促进依赖于PTK激酶活性的细胞外基质信号转导，进而影响细胞的黏附、运动与迁移。
2.粘着斑激酶（focal adhesion kinase,FAK）是整合蛋白介导的信号转导中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化；为信号分子的FAK,还与细胞内其他信号转导通路存在串话（crosstalk）,直接参与了细胞多种功能的调节。
3.尽管FAK的确切功能尚不清楚,但若干实验均提示FAK可能有两个作用,一是在细胞铺展和移动时,FAK参与粘着斑形成和调节；二是FAK参与信号转导过程,以告知细胞核其细胞已锚定了。近年有关FAK在细胞凋亡中的作用也业已肯定。