- 面议
参考价：
起订量：
M0049 心肌黄酶/黄递酶/硫辛酰胺脱氢酶/二氢硫辛酰胺脱氢酶/二氢硫辛酸脱氢酶/Lipoamid
- 型号
- M0049
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详细信息
| M0049 | 心肌黄酶|黄递酶 | BR | 源叶 |
英文名称：Diaphorase from clostridium kluyveri
其他名称：黄递酶；硫辛酰胺脱氢酶；二氢硫辛酰胺脱氢酶；二氢硫辛酸脱氢酶
CAS号：9001-18-7
分子量：102000～110000
活力：≥25units/mg
活力定义： 1 unit reduces 1umole of DCPIP per minute at25℃，pH8.5（在25℃，pH8.5，1分钟还原1μmol 2，6-二氯酚靛酚的酶量为1单位）
描述：A lyophillized powder,dialyzed to remove pyridine nucleotides
性状：浅棕黄色冻干结晶或呈现荧光的黄绿色粉末，zui适pH值8.5。N-乙基顺丁烯二酰亚胺对其有抑制作用
用途：用于生化研究，测定乳酸脱氢酶。除了能催化二氢硫辛酰胺或二氢硫辛酸脱氢，分别成为硫辛酰胺和硫辛酸外，也可催化人工电子受体还原，如某些染料、铁氰酸盐、醌等。脱去的两个氢能将FAD（黄素腺嘌呤二核苷酸）还原为FADH2，再传递给NAD+（烟酰胺腺嘌呤二核苷酸+）而形成NADH和H+，该酶主要用于生化试剂
保存：2～8℃避光保存
其他名称：黄递酶；硫辛酰胺脱氢酶；二氢硫辛酰胺脱氢酶；二氢硫辛酸脱氢酶
CAS号：9001-18-7
分子量：102000～110000
活力：≥25units/mg
活力定义： 1 unit reduces 1umole of DCPIP per minute at25℃，pH8.5（在25℃，pH8.5，1分钟还原1μmol 2，6-二氯酚靛酚的酶量为1单位）
描述：A lyophillized powder,dialyzed to remove pyridine nucleotides
性状：浅棕黄色冻干结晶或呈现荧光的黄绿色粉末，zui适pH值8.5。N-乙基顺丁烯二酰亚胺对其有抑制作用
用途：用于生化研究，测定乳酸脱氢酶。除了能催化二氢硫辛酰胺或二氢硫辛酸脱氢，分别成为硫辛酰胺和硫辛酸外，也可催化人工电子受体还原，如某些染料、铁氰酸盐、醌等。脱去的两个氢能将FAD（黄素腺嘌呤二核苷酸）还原为FADH2，再传递给NAD+（烟酰胺腺嘌呤二核苷酸+）而形成NADH和H+，该酶主要用于生化试剂
保存：2～8℃避光保存
| M0049 | 心肌黄酶|黄递酶 | BR | 进口原装 |
英文名称：Diaphorase from clostridium kluyveri
其他名称：黄递酶；硫辛酰胺脱氢酶；二氢硫辛酰胺脱氢酶；二氢硫辛酸脱氢酶
CAS号：9001-18-7
分子量：102000～110000
活力：5-20 units/mg protein （biuret）
活力定义： One unit of either "diaphorase" or "lipoyl" dehydrogenase will oxidize 1.0 μmole of β-NADH per min at pH 7.5 at 25 °C, with the corresponding reduction of 2,6-dichlorophenolindophenol
描述：The name "Diaphorase" has been loosely applied to several enzymes which catalyze the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Many different assay procedures and "units" are used. Diaphorases which are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase （β-NADH specific） as well as lipoic and lipoamide dehydrogenase activities. It is reported to be a single protein. However, Massey reports that "diaphorase" is probably a denatured lipoamide dehydrogenase. Pre-incubation of the pig heart preparation with Cu2+ reduces the lipoamide dehydrogenase activity and proportionay increases the β-NADH diaphorase activity. In our laboratory, we have demonstrated this copper effect to some degree on the pig heart enzyme, but no appreciable effect was observed on the Clostridium kluyveri or torula yeast preparations. The lipoamide dehydrogenase:diaphorase ratio is a measure of the denaturation
性状：浅棕黄色冻干结晶或呈现荧光的黄绿色粉末，zui适pH值8.5。N-乙基顺丁烯二酰亚胺对其有抑制作用
用途：用于生化研究，测定乳酸脱氢酶。除了能催化二氢硫辛酰胺或二氢硫辛酸脱氢，分别成为硫辛酰胺和硫辛酸外，也可催化人工电子受体还原，如某些染料、铁氰酸盐、醌等。脱去的两个氢能将FAD（黄素腺嘌呤二核苷酸）还原为FADH2，再传递给NAD+（烟酰胺腺嘌呤二核苷酸+）而形成NADH和H+，该酶主要用于生化试剂
保存：2～8℃避光保存
其他名称：黄递酶；硫辛酰胺脱氢酶；二氢硫辛酰胺脱氢酶；二氢硫辛酸脱氢酶
CAS号：9001-18-7
分子量：102000～110000
活力：5-20 units/mg protein （biuret）
活力定义： One unit of either "diaphorase" or "lipoyl" dehydrogenase will oxidize 1.0 μmole of β-NADH per min at pH 7.5 at 25 °C, with the corresponding reduction of 2,6-dichlorophenolindophenol
描述：The name "Diaphorase" has been loosely applied to several enzymes which catalyze the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Many different assay procedures and "units" are used. Diaphorases which are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase （β-NADH specific） as well as lipoic and lipoamide dehydrogenase activities. It is reported to be a single protein. However, Massey reports that "diaphorase" is probably a denatured lipoamide dehydrogenase. Pre-incubation of the pig heart preparation with Cu2+ reduces the lipoamide dehydrogenase activity and proportionay increases the β-NADH diaphorase activity. In our laboratory, we have demonstrated this copper effect to some degree on the pig heart enzyme, but no appreciable effect was observed on the Clostridium kluyveri or torula yeast preparations. The lipoamide dehydrogenase:diaphorase ratio is a measure of the denaturation
性状：浅棕黄色冻干结晶或呈现荧光的黄绿色粉末，zui适pH值8.5。N-乙基顺丁烯二酰亚胺对其有抑制作用
用途：用于生化研究，测定乳酸脱氢酶。除了能催化二氢硫辛酰胺或二氢硫辛酸脱氢，分别成为硫辛酰胺和硫辛酸外，也可催化人工电子受体还原，如某些染料、铁氰酸盐、醌等。脱去的两个氢能将FAD（黄素腺嘌呤二核苷酸）还原为FADH2，再传递给NAD+（烟酰胺腺嘌呤二核苷酸+）而形成NADH和H+，该酶主要用于生化试剂
保存：2～8℃避光保存
