产品编号bs-0916R
英文名称Uromucoid
中文名称尿调节蛋白抗体
别    名Uromodulin; ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; MGC14034; TAMM-HORSFALL; THGP; THP; UMOD; urehr4; Uromodulin; uromucoid; UROM_MOUSE; UROM_HUMAN.  
规格价格0.1ml/980元 购买    0.2ml/1580元 购买    
说 明 书0.1ml  0.2ml  
研究领域细胞生物  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow, 
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量68kDa
细胞定位细胞膜 
性    状Lyophilized or Liquid
浓    度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human MCKD2
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
生 产 商BIOSS
PubMedPubMed
尿调节蛋白抗体产品介绍background:
This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq]

Function:
Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial (By similarity). 
Uromodulin, secreted form: Secreted into urine after proteolytically cleaveage. Into the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and modulates formation of supersaturated salts and their crystals.

Subcellular Location:
Apical cell membrane; Lipid-anchor, GPI-anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane. Note=Only a small fraction is sorts to the basolateral pole of tubular epithelial cells compared to apical localization. 
Uromodulin, secreted form: Secreted.

Post-translational modifications:
N-glycosylated.

Similarity:
Contains 3 EGF-like domains. 
Contains 1 ZP domain.

SWISS:
Q91X17

Gene ID:
7369

Database links:
Entrez Gene: 7369 Human
Omim: 191845 Human
SwissProt: P07911 Human
SwissProt: Q5R5C1 Orangutan
Unigene: 654425 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-UMOD Polyclonal Antibody, Unconjugated(bs-0916M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining

Blank control: 293T (blue). 
Primary Antibody:Rabbit Anti-Uromucoid antibody(bs-0916R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; 
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); 
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-0916R, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.