产品编号bs-2616R
英文名称IL18R Beta
中文名称白细胞介素-18受体β链抗体
别    名IL-18R Beta; Interleukin-18 receptor beta; Accessory protein like; IL18R Beta; AcPL; IL 18RacP; IL 1R accessor protein like; IL 1R7; IL 1RacPL; IL18Rbeta; Interleukin 18 receptor accessory protein; IL18RAP; Interleukin-18 receptor accessory protein-like; IL-18R-beta; IL-18Rbeta; Accessory protein-like; AcPL; Interleukin-1 receptor 7; IL-1R-7; IL-1R7; CD218 antigen-like family member B; CD218b; I18RA_HUMAN.  
JournalPMIDIFApplicationCellular signalling (2013)235414424.3040IP/IB
规格价格0.1ml/980元 购买    0.2ml/1580元 购买    
说 明 书0.1ml  0.2ml  
研究领域细胞生物  免疫学  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Horse, 
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:50-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量68kDa
细胞定位细胞膜 
性    状Lyophilized or Liquid
浓    度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human IL-18R beta
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
生 产 商BIOSS
PubMedPubMed
白细胞介素-18受体β链抗体产品介绍background:
IL18R Beta is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and IL18R Beta is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18.

Function:
Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.

Subunit:
Together with IL18R1, probably belongs to the IL-18 receptor complex. Seems not to interact directly with IL-18.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.

Similarity:
Belongs to the interleukin-1 receptor family. 
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. 
Contains 1 TIR domain.

SWISS:
O95256

Gene ID:
8807

Database links:
Entrez Gene: 8807 Human
Entrez Gene: 16174 Mouse
Omim: 604509 Human
SwissProt: O95256 Human
SwissProt: Q9Z2B1 Mouse
Unigene: 158315 Human
Unigene: 20466 Mouse
Unigene: 392521 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

白细胞介素-18受体β链抗体产品图片
Tissue/cell: human pulmonitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-IL-18R Beta/IL1R7/CD218b Polyclonal Antibody, Unconjugated(bs-2616R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Cell: (mo)Splenocyte(2% BSA at 4°blocked for 30 minutes.1% Paraformaldehyde fixed for 30 minutes).
Concentration:1:100. 
Incubation: 40 minutes. 
Host/Blank: Splenocytes. 
Flow cytometric analysis of Rabbit Anti-IL18R Beta antibody (bs-2616R)(green) compared with control in the absence of primary antibody (blue) followed by splenocytes.
Secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC).

Sample:Spleen (Mouse)Lysate at 40 ug
Primary: Anti-IL18R Beta(bs-2616R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 68kD