起订量:
15032350 illumina NXTR XT样本制备试剂盒 *
中级会员第8年
生产厂家上海易汇生物科技有限公司,是一家集研发生产、销售为一体的生物企业,公司专注于生命科学及生物技术领域,专业提供医疗耗材,分子试剂等产品。自成立以来易汇秉承“专注生命科学,服务于客户,诚信平等”为理念。本公司推出“Eaivelly”品牌耗材,产品符合GMP要求的十万级无尘制造车间,产品采用进口塑胶医用级聚苯乙烯材料,所有产品均无DNase和RNase。目前已拥有PCR管、PCR板、培养皿、细胞培养板、血清移液管、针式过滤器、离心管、巴氏吸管等产品。Eaivelly以“品质为主导,科学创新”为宗旨,为生命科学尽心尽责。
illumina NXTR XT样本制备试剂盒 *
illumina NXTR XT样本制备试剂盒 *
illumina 15032350
illumina 15032350 NXTR XT样本制备试剂盒
NXTR XT样本制备试剂盒(24样本 第一盒)
Nextera XT Library Prep kit 96 Samples(BOX 1of2) 15032354
NXTR XT标签试剂盒V2(套件A 96标签 192样本)15052163
Next-Generation sequencing
Total RNA was extracted from each patient’s plasma sample, and cDNA synthesis followed. The QIAamp Viral RNA Mini Kit (Cat. #52904, Qiagen, Gaithersburg, MD) and the SuperScript�6�4 IV First-Strand Synthesis System (Cat. # 18091050, Invitrogen, Carlsbad, CA) were used respectively and according to manufacturers’ instructions. Multiple sets of HIV-1 specific primer pairs were used to target and amplify using polymerase-chain-reaction (PCR) the protease (PR), reverse transcriptase (RT), integrase (int), and envelope (env) HIV-1 genes (~43% of genome)36. Library preparation was completed with Nextera XT Library Prep (Cat. # 15032350, Illumina, Dan Diego, CA). Samples were then sequenced on eight runs on an Illumina MiSeq platform using the MiSeq v2 (300 cycles) chemistry (Cat. # MS-102–2002, Illumina). Both library prep and sequencing were completed according to the manufacturer’s instructions. All DNA sequence files are available from the GenBank database under SRA accession: PRJNA517147.