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因美纳 20009584 Illumina ISeq 100 i1试剂卡盒V2 300个循环
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Illumina ISeq 100 i1试剂卡盒V2 300个循环
Illumina ISeq 100 i1试剂卡盒V2 300个循环
Illumina 20009584
因美纳
ISeq 100 i1试剂卡盒V2 300个循环
ISeq 100 i1Reagent Cartridge V2 300cycles
MADE IN USA
Illumina,20031371,TruSeq Index Plate Fixture Kit (2 fixtures)20009555/20009584
An iSeq v2 reagent cartridge (20031371, Illumina) is thawed in a water bath at room temperature for 1 h; the flow cell is inserted; and the kit is kept at room temperature. The LAMP-Seq library and the N-100 random-index library are diluted to 2 nM each in water. Next, 1 µl of the combined library is mixed with 150 µl of Tris pH 8.5. Then, 20 µl of the final dilution is loaded into the reagent cartridge and sequenced according to the manufacturer’s instruction using the following cycle numbers:
100 cycles read-1
8 cycles index-1
8 cycles index-2