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AT0025 Anti-His tag小鼠单克隆抗体

型号
AT0025
参数
供货周期:现货 规格:100UL 货号:AT0025 主要用途:His标签融合蛋白的检测(WB,IHC,IF,IP,CoIP)
上海维景生物科技有限公司

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上海维景生物科技有限公司是一家专注生命科学领域的企业,拥有强大的资金背景支持和专业的团队等资源。公司面向全国市场,迅速发展,广纳英才,现已建成专业生命科学产品销售团队,其中大部份是在生命科学市场上工作多年,有丰富的销售经验,也有良好的客户资源,销售渠道全面覆盖全国范围大部分工业以及科研客户,团队中免疫学及相关专业硕士以上学历人士达80%,有较强的技术支持能力,在针对专业技术服务中能够提供完善周到的专业化服务,对客户在使用产品过程中提出的问题能满意答复,并可及时传递较早的产品技术资料信息。  

上海维景生物是一家面向全国以专业从事免疫学实验试剂和抗体试剂代理销售为主的公司,服务对象主要为大学研究所科研人员、大型制药公司等。

同时公司还是美国Engibody品牌的全国总代理Engibody公司产品专注于标签抗体、内参抗体及标签抗体偶联beads这一领域。其开发的标签抗体偶联agarose beads(affinity gel)在科研以及临床研究中享有很高的声誉,更借助Engibody在标签抗体、内参抗体领域的优势,成为WB,IP,Co-IP全套试剂及试剂盒的专业供应商。


维景生物是一家专注标签抗体偶联beads(affinity gel),标签抗体,HRP直标标签抗体,内参抗体等抗体销售,及WB,IP,Co-IP全套试剂及试剂盒的高科技生物公司。

 

 

 

详细信息

产品名称:Anti-His tag小鼠单克隆抗体


货号: AT0025

品牌:Engibody


简介:His标签抗体,亲和力高,特异性好,适用于检测细菌、酵母、昆虫细胞或哺乳动物细胞表达的包含His标签的融合蛋白,无论his标签处于融合蛋白的N端,C端还是中间,都可以


一管搞定全部实验(WB,IHC,ICC,IF及IP/CoIP)


The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.


As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.


Description

Mouse monoclonal antibody to 6X His tag - Epitope Tag Antibody


Reactivity

All Species Expected


Tested applications

WB    : 1/1000-1/5000.

IHC    : 1/200-1/3000.

ICC/IF    : 1/200-1/1000.

IP/CoIP    : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.


Specificity

This antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.


Immunogen

Synthetic peptide: HHHHHH conjugated to KLH.


Clonality

Monoclonal, clone number: 2B6


Isotype

Mouse IgG1


Form

Liquid,100 μg at 1mg/mL

Mouse monoclonal immunoglobulin in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 50% glycerol with 0.1mg/mL BSA (IgG, protease free) as a stabilizer and 0.02% sodium azide as preservative.


Storage instruction

Store at -20°C, Avoid freeze / thaw cycle.


Database links

Sequence: HHHHHH


Host

Mouse



H-H-H-H-H基序用作许多重组蛋白上的标签,以便于纯化。抗体识别与转染或转化细胞中靶蛋白的氨基或羧基末端融合的His标签。


描述

6X His标签小鼠单克隆抗体


反应性

所有物种表达的带有His标签的融合蛋白


已测试的应用实验


WB:1/1000-1/5000。


IHC:1/200-1/3000。


ICC/IF:1/200-1/1000。


IP/CoIP:1/100-1/500。


尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。


特异性

该抗体检测含有6xHis表位标签的重组蛋白。抗体识别融合到转染细胞中靶蛋白的氨基或羧基末端的6xHis标签。


免疫原

合成肽:与KLH偶联的HHHHHH。


克隆性

单克隆,克隆号:2B6


同型对照

小鼠IgG1


类型

液体,100μg,1mg/mL

小鼠单克隆免疫球蛋白,磷酸盐缓冲盐水(PBS),pH 7.3(+/-0.1),50%甘油,0.1mg/mL BSA(IgG,无蛋白酶)作为稳定剂,0.02%防腐剂。


存储说明

-20°C下,避免冷冻/解冻循环。


序列:

HHHHHH


抗体来源宿主

小鼠

638024968289216025791.png

Lane 1: lysates prepared from HEK-293 cells transfected with his-tagged protein at 1:4000 dilution


Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)


638024968289684302836.jpg



Western Blot:12 % SDS-PAGE 6x His antibody at 1:5000.

A) 2 µg GST-recombinant protein

B) 2 µg His-recombinant protein

C) 1 µg His-recombinant protein

D) 0.5 µg His-recombinant protein

E) 0.1 µg His-recombinant protein.

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)


638024968348205589121.jpg


Immunocytochemistry/Immunofluorescence:  - Analysis using Anti-6 x Histidine Epitope Tag Antibody and goat anti mouse IgG conjugated DyLight 488.

Staining of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Anti-6x-His Epitope Tag mouse monoclonal antibody at a dilution of 1:500 for at least 1 hour at room temperature, then using goat anti mouse IgG conjugated DyLight 488. Nuclei (blue) were stained with Hoechst 33342 dye.


638024968288904813803.jpg

Immunoprecipitation and Western Blot: His Tag Antibody - Analysis of cell lysate/extract of His-fused gene transfected HeLa cells
A. 20µg lysate/extract of transfected HeLa cells
B. Control with 1:1000 of mouse normal IgG
C. Immunoprecipitation of His-fused protein by 1:500 of 6X His tag Mouse mAb, the immunoprecipitated His-fused protein was detected by 6X His tag Rabbit pAb diluted at 1:3000.


Recommended immunoprecipitation antibody for IP: Anti-His tag Mouse mAb (Engibody, Catalog number:AT0025)
Recommended protein A/G beads: Protein A/G Agarose Beads (Engibody, Catalog number: IF0001), or Protein A/G Magnetic Beads (Engibody, Catalog number: IF0002)


Recommended detection antibody for downstream WB: Anti-His tag Rabbit mAb (Engibody, Catalog number:AT0042), so that the heavy and light chains from immunoprecipitation antibody can't be seen in WB.
Recommended secondary antibody for downstream WB: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)
Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)




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产品参数

供货周期 现货
规格 100UL
货号 AT0025
主要用途 His标签融合蛋白的检测(WB,IHC,IF,IP,CoIP)
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