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AT0028 Anti-GFP小鼠单克隆抗体
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上海维景生物科技有限公司是一家专注生命科学领域的企业，拥有强大的资金背景支持和专业的团队等资源。公司面向全国市场，迅速发展，广纳英才，现已建成专业生命科学产品销售团队，其中大部份是在生命科学市场上工作多年，有丰富的销售经验，也有良好的客户资源，销售渠道全面覆盖全国范围大部分工业以及科研客户，团队中免疫学及相关专业硕士以上学历人士达80%，有较强的技术支持能力，在针对专业技术服务中能够提供完善周到的专业化服务，对客户在使用产品过程中提出的问题能满意答复，并可及时传递较早的产品技术资料信息。
上海维景生物是一家面向全国以专业从事免疫学实验试剂和抗体试剂代理销售为主的公司，服务对象主要为大学研究所科研人员、大型制药公司等。
同时公司还是美国Engibody品牌的全国总代理，Engibody公司产品专注于标签抗体、内参抗体及标签抗体偶联beads这一领域。其开发的标签抗体偶联agarose beads（affinity gel）在科研以及临床研究中享有很高的声誉，更借助Engibody在标签抗体、内参抗体领域的优势，成为WB,IP,Co-IP全套试剂及试剂盒的专业供应商。
维景生物是一家专注标签抗体偶联beads（affinity gel），标签抗体，HRP直标标签抗体，内参抗体等抗体销售,及WB,IP,Co-IP全套试剂及试剂盒的高科技生物公司。
详细信息
产品名称：Anti-GFP小鼠单克隆抗体
货号： AT0028
品牌：Engibody
简介：GFP标签抗体，一管搞定全部实验（WB，IHC，ICC，IF及IP/CoIP）
The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization.
Description
Mouse monoclonal antibody to GFP
Reactivity
green fluorescent protein (GFP), Enhanced green fluorescent protein (EGFP)
Tested applications
WB : 1/2000-1/10000.
IHC : 1/200-1/5000.
ICC/IF : 1/200-1/2000.
IP/CoIP : 1/100-1/500.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Specificity
This antibody detects GFP, EGFP -tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect RFP-tagged proteins.
Please note that the GFP, EGFP tags add approximately 27 kDa to the molecular weight of the fusion protein.
The antibody recognizes the GFP, EGFP tag, which is fused to either the amino-terminus or carboxy-terminus of the target protein.
Immunogen
a Green Fluorescent Protein (GFP) recombinant protein.
Clonality
Monoclonal, clone number: 7C5
Isotype
Mouse IgG2a
Form
Liquid, 100 μg at 1mg/mL
Mouse monoclonal antibody in 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.
Storage instruction
Store at -20°C, Avoid freeze / thaw cycle.
Host
Mouse
绿色荧光蛋白（GFP）在水母Aequorea中充当生物发光能量转移受体，其最大吸收395nm处的光。GFP作为一种融合蛋白已成为一种非常有用的工具，可报告基因表达、追踪细胞谱系和确定亚细胞蛋白定位。
描述
抗GFP的小鼠单克隆抗体
反应性
增强型绿色荧光蛋白（EGFP）
已测试的应用实验
WB:1/2000-1/10000。
IHC:1/200-1/5000。
ICC/IF:1/2000-1/2000。
IP/CoIP:1/100-1/500。
尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。
特异性
这种抗体检测细胞或大肠杆菌中外源表达的GFP、EGFP标记蛋白。该抗体不能检测RFP标记的蛋白质。
请注意，GFP、EGFP标签使融合蛋白的分子量增加了约27kDa。
抗体识别GFP，即EGFP标签，融合到目标蛋白的氨基末端或羧基末端。
免疫原
绿色荧光蛋白（GFP）重组蛋白。
克隆性
单克隆，克隆号：7C5
同型对照
小鼠IgG2a
类型
液体，100μg，1mg/mL
小鼠单克隆抗体在10 mM磷酸盐缓冲盐水（PBS）中，pH 7.4，50%甘油，1 mg/mL（0.1%w/v）BSA（不含IgG，不含蛋白酶）作为稳定剂，0.02%（w/v）防腐剂。
存储说明
-20°C下，避免冷冻/解冻循环。
抗体来源宿主
小鼠
Western Blot: GFP Antibody
Lane 1: HEK293T lysate 50µg (transfected pEGFP-N1-POI).
Lane 2: HEK293T lysate 50µg (transfected pEGFP -C1-POI).
Lane 3: Hela lysate 50µg (transfected pEGFP -N1-POI).
Primary antibody: GFP antibody at 1:5000
Observed band size: 48 kDa
Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number: AT0098)
Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)
Immunocytochemistry / Immunofluorescence:
GFP Antibody(AT0028) - GFP-transfected 293T cells.
mCherry antibody (AT0037) labels mCherry tagged protein
Nuclei were counterstained with DAPI.
Immunoprecipitation: Anti-GFP tag Mouse mAb (Engibody, Catalog number:AT0028)
Analysis of 400 µg cell lysate/extract of GFP-fused gene transfected 293T cells
Lane 1: 30 µg lysate/extract of transfected 293T cell
Lane 2: Immunoprecipitation of GFP-fused protein by 1:500 of GFP tag Mouse mAb
Lane 3: Control with 1:500 of normal mouse IgG
Recommended immunoprecipitation antibody for IP: Anti-GFP tag Mouse mAb (Engibody, Catalog number:AT0028)
Recommended protein A/G beads: Protein A/G Agarose Beads (Engibody, Catalog number: IF0001), or Protein A/G Magnetic Beads (Engibody, Catalog number: IF0002)
Recommended detection antibody for downstream WB: Anti-GFP tag mouse mAb (Engibody, Catalog number:AT0028)
Recommended secondary antibody for downstream WB: MOU-IP Anti-mouse IgG for IP (HRP) (Engibody, Catalog number:AT9204), so that the heavy and light chains from immunoprecipitation antibody can't be seen in WB.
