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详细信息

QIAprep Spin Miniprep Kit

纯化分子生物学级别纯度质粒DNA，zui高可达 20 μg

数分钟内即可获得即用型质粒DNA
非树脂技术，无需酚抽提
分子生物学级别纯度的质粒DNA，产量重现性高

Product Details

Principle

QIAprep spin columns contain a unique silica gel membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediay ready to use - there is no need to precipitate, concentrate, or desalt.

Procedure

Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure （see figure "The QIAprep procedure"）. First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silicagel membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.

The QIAprep Spin MiniPrep Kit enables purification of up to 20 ug molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges or on vacuum manifolds （see figure "QIAprep Spin Column handling options"）. The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 plus, QIAvac 6S withQIAvac Luer Adapters, or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.

Characteristics
Format	Spin columns
Purification module	QIAprep spin columns
Throughput	1–24 samples
Preparation time	24 minipreps in 30 minutes
Equipment required	Microcentrifuge or vacuum manifold; fully automatable using the QIAcube
Lysate clearing	Centrifugation

Specifications
Capacity of column reservoir	800 µl
Minimum elution buffer volume	50 µl
Culture volume for high-copy plasmids	1–5 ml
Culture volume for low-copy plasmids/cosmids	1–10 ml

Applications

QIAprep Miniprep Kits provide reproducible yields of high-purity DNA suitable for use in most applications, including:

Automated fluorescent sequencing, including capillary sequencing
Radioactive sequencing
Restriction digestion （see figure "Complete digestion with various restriction enzymes"）
Screening
Ligation and transformation
Transfection of robust cells

In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.