DIG Oligonucleotide 3'-End Labeling Kit, 2nd generation
产品包装: 1 kit for 25 labeling reactions 100 pmol of oligonucleotides per assay corresponding to 1 μg of a 30-mer oligonucleotide
产品组成成份:
- Reaction Buffer 5x, 100 µl
- CoCl2 Solution, 100 µl, 25 mM
- DIG-ddUTP Solution, 25 µl, 1 mM
- Recombinant Terminal Transferase, 25 µl, [400 U/µl]
- Control Oligonucleotide, unlabeled, 25 µl, [20 pmol/µl]
- Oligonucleotide, DIG-ddUTP labeled, 100 µl, [2.5 pmol/µl]
- Control DNA, 20 µl, [2.5 pmol/µl] pUC 18 DNA, supercoiled
- Glycogen Solution, 50 µl, [20 mg/ml]
- DNA Dilution Buffer, 1 ml, [50 µg/ml] fish sperm DNA
试剂盒原理:
One DIG-ddUTP molecule is added to the 3'-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction.
: 61288195 Mr Shen