- ¥50341起订
参考价：
起订量：
Roche03353583910原装 罗氏地高辛标记检测试剂盒
- 型号
- Roche03353583910原装
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广州市齐云生物技术有限公司位于广州市越秀区，是一家专业从事科研用生化试剂及相关产品销售的公司。公司产品涉及生化试剂、标准品、ELISA试剂盒、RT-PCR相关试剂、基因检测试剂、农业残留检测试剂等众多领域，致力于为生物企业、科研院所、医疗机构提供*的生化试剂、生物产品服务。
公司自成立以来，与国内外企业，科研院校和多家代理商建立了良好的合作关系，是国内产品系列多，品种全，质量优的生物产品和生化试剂供应商。能够满足各生物企业、科研院所、医疗机构的各种需求，能*提供一站式试剂供应服务。专业企业，专业品质，专业服务，“广州齐云，试剂专家”，公司立志于做广州地区*的科研试剂经销商
主要代理品牌：
详细信息
| Catalog # | Pack size | Qty | Price | Options |
|---|---|---|---|---|
| 1 kit for 25 tailing reactions 100 pmol oligonucleotide per assay corresponding to 1 μg of a 30-mer oligonucleotide | 5034.00 |
一、使用原理：
Oligonucleotides, tailed with DIG-dUTP, are used for DNA or RNA hybridization in membrane blots or in situ hybridization.
二、产品优点：
- Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
- Fast hybridization kinetics, due to the small size of oligonucleotides
- Single-stranded probes, no renaturation during hybridization
- Sequence can be designed according to the experiment
- Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells
三、产品描述：
Tailing of oligonucleotides at the 3'-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides （tail length range: 10 – 100）.
四、试剂盒组成组分:
- Reaction Buffer, 5x, 100 µl
- CoCl2 Solution, 100 µl, 25 mM
- DIG-dUTP Solution, 25 µl, 1 mM
- dATP Solution, 25 µl, 10 mM
- Recombinant Terminal Transferase, 25 µl, [400 U/µl]
- Control Oligonucleotide, unlabeled, 25 µl, [20 pmol/µl]
- Oligonucleotide, DIG-dUTP/dATP tailed, 20 µl, [2.5 pmol/µl]
- Control DNA, 20 µl, [0.25 mg/ml]
- Glycogen Solution, 50 µl, [20 mg/ml]
- DNA Dilution Buffer, 3 x 1 ml, [50 µg/ml] fish sperm DNA
- poly（A） Solution, 1 ml, [10 mg/ml]
五：试剂盒检测原理：
DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide（s）.
六：：，61288195 Mr SHen
