- ¥62001起订
起订量：
Roche11175025910 地高辛标记DNA检测试剂盒（DIG RNA Labeling Kit （SP6/T7） ）
- 型号
- Roche11175025910
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广州市齐云生物技术有限公司位于广州市越秀区，是一家专业从事科研用生化试剂及相关产品销售的公司。公司产品涉及生化试剂、标准品、ELISA试剂盒、RT-PCR相关试剂、基因检测试剂、农业残留检测试剂等众多领域，致力于为生物企业、科研院所、医疗机构提供*的生化试剂、生物产品服务。
公司自成立以来，与国内外企业，科研院校和多家代理商建立了良好的合作关系，是国内产品系列多，品种全，质量优的生物产品和生化试剂供应商。能够满足各生物企业、科研院所、医疗机构的各种需求，能*提供一站式试剂供应服务。专业企业，专业品质，专业服务，“广州齐云，试剂专家”，公司立志于做广州地区*的科研试剂经销商
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详细信息
DIG RNA Labeling Kit （SP6/T7） | |
| For RNA labeling with digoxigenin-UTP by in vitro transcription with SP6 and T7 RNA polymerase. | |
| Catalog # | Pack size | Qty | Price | Options |
|---|---|---|---|---|
| 11175025910 | 1 kit for 2× 10 labeling reactions | 6200 |
Application
RNA labeling with DIG-11-UTP by in vitro transcription with SP6 and T7 RNA Polymerases. DIG-labeled “run off” transcripts are synthesized with high efficiency and can be used in northern blots, Southern blots, in situ hybridization, plaque or colony lifts, and RNase protection experiments.
Contents
- pSPT18 DNA, 40 µl, 0.25 mg/ml
- pSPT19 DNA, 40 µl, 0.25 mg/ml
- Control DNA 1, pSPT18-Neo, 20 µl, 0.25 mg/ml cleaved with Pvu II
- Control DNA 2, pSPT19-Neo, 20 µl, 0.25 mg/ml cleaved with Pvu II
- DIG-labeled Control RNA, 100 µl, 100 ng/µl DIG-labeled “antisense” neo RNA dimethyldicarbonate treated double-distilled water
- Unlabeled Control RNA, 20 µl, 200 µg/ml neo poly （A） “sense” RNA in dimethyldicarbonate treated double-distilled water
- NTP Labeling Mixture, 40 µl, 10x conc. 10 mM ATP, CTP, GTP, 6.5 mM UTP, 3.5 mM DIG-11-UTP, pH 7.5
- Transcription Buffer, 40 µl, 10x conc.
- DNase I, RNase-free, 40 µl, 10 U/µl
- Protector RNase Inhibitor, 20 µl, 20 U/µl
- SP6 RNA Polymerase, 20 µl, 20 U/µl
- T7 RNA Polymerase, 20 µl, 20 U/µl
Quality
Test principle: The DNA template to be transcribed is cloned into the polylinker site of appropriate transcription vectors, which contain promoters for SP6 and/or T7 RNA Polymerases. After linearization at a suitable site, RNA is transcribed in the presence of DIG-UTP. Under standard conditions, approximay 10 µg of full-length DIG-labeled RNA is transcribed from 1 µg template.
