B27细胞培养添加剂invitrogen/Gibco(货号17504-044)说明书

B27细胞培养添加剂invitrogen/Gibco(货号17504-044)说明书

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2018-09-12 13:57:35
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上海恪敏生物科技有限公司

上海恪敏生物科技有限公司

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产品简介

B-27® 添加剂是无血清添加剂,用于海马神经元和其他中枢神经系统(CNS)神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体,可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用,用于神经细胞培养而不需要再添加饲养层细胞。

详细介绍

 

描述

B-27® 添加剂是无血清添加剂,用于海马神经元和其他中枢神经系统(CNS)神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体,可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用,用于神经细胞培养而不需要再添加饲养层细胞。

B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用,我们提供 其他配方的B-27® 添加剂.

质量控制检测 
B-27® 添加剂 进行了18天妊娠的原代大鼠(Sprague Dawley) 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。 

仅供研究使用。除非另有说明,不得用于任何动物或人类治疗或诊断。

详细说明

常用规格

Cell Type:

Neuronal Cells 

Product Size:

10 ml 

Classification:

Serum-Free 

Other Additives:

Vitamin A, 

Antioxidant Cocktail, 

Insulin 

Purity or Quality Grade:

Cell Culture Grade 

Green Features:

Sustainable packaging 

Shipping Condition:

Dry Ice 

Regulatory Statement:

For Research Use Only. Not for use in diagnostic procedures. 

引文和参考文献 (17)

  • D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

作者
Congar P; Bergevin A; Trudeau LE
杂志
J Neurophysiol (2002) 87:1046-1102
产品用途
Using a culture system in which isolated DAergic neurons establish self-innervating synapses ("autapses") that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents (EPSCs). 
ID:
PN64425
  • Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

作者
Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;
杂志
Neuron (2002) 33:789-804
产品用途
Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator. 
ID:
PN47418
  • Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.

作者
Kabos Peter; Kabosova Andrea; Neuman Toomas;
杂志
J Biol Chem (2002) 277:8763-8769
产品用途
Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized (0.02 mg/ml trypsin in versene (Invitrogen) at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement (Invitrogen). Cells were washed with PBS (Invitrogen)  
ID:
PN63471
  • Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

作者
Lesuisse Christian; Martin Lee J;
杂志
J Neurobiol (2002) 51:9-23
产品用途
A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. 
ID:
PN60461
  • An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

作者
Joseph-Bravo Patricia; Pérez-Martínez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;
杂志
Brain Res Brain Res Protoc (2002) 9:93-104
产品用途
Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties 
ID:
PN62028

按细胞系排列的引文

细胞系名称 有机体类型 组织
SH-Sy5Y Human Brain
Fetal neurons Rat Brain
Neurons (post-natal/adult) Rat Brain
Neurons (superior cervical ganglia - SCG) Rat Brain
Neurons (cortical) Mouse Other
Neural Stem Cells Rat Brain
 
 
 
主营:生化试剂、抗体、重组蛋白、细胞生物、分子生物、蛋白研究、微生物、Elisa试剂盒、耗材、标准品、 标准溶液……
abcam、ABI、Axygen、corning、信号转导抗体、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等进口品牌……
以下为本公司常备现货产品

 

*部分 invitrogen 原装产品

 

 

品牌 货号 名称 规格
invitrogen 13778-075 Lipofectamine RNAiMAX 0.75ml
invitrogen 13778-150 Lipofectamine RNAiMAX 1.5ml
invitrogen 11668-027 Lipofectamine 2000 Reagent 0.75ml
invitrogen 11668-019 Lipofectamine 2000 Reagent 1.5ml
invitrogen 15596-026 TRlzol ®Reagent 100ml
invitrogen 15596-018 TRlzol ®Reagent 200ml
invitrogen 10296-010 Trizol ®  LS Reagent 100ml
invitrogen 10296-028 Trizol ®  LS Reagent 200ml
invitrogen 17504-044 B-27(黄颜色) 10ml
invitrogen 12587-010 B-27(黄颜色)不含维生素A 10ml
invitrogen 17502-048 N-2  (粉红颜色) 5ml
invitrogen 18064-014 SuperScript® II Reverse Transcriptase 10,000U
invitrogen 18080-044 SuperScript® III Reverse Transcriptase 10,000U

 

 

第二部分 HyClone胎牛血清

 

 

品牌 货号 名称 规格
HyClone SH30370.03 Australian Standard Fetal Bovine Serum 500ml
HyClone SH30406.02 FOETAL BOVINE SERUM 500ml
HyClone SH30401.01 New Zealand Calf Serum 500ml
HyClone SH30066.02 FetalClone® II 100ml
HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml
HyClone SH30118.02 Newborn Bovine Calf Serum, U.S. Origin 100ml
HyClone SH30070.02 FETAL BOVINE SERUM 100ml
HyClone SH30087.02 COSMIC CALF® SERUM 100ml
HyClone SH30070.03E FETALBOVINESERUM CHARACTERIZSED 500ml
100ml
HyClone SH30070.03 FETAL BOVINE SERUM 500ml
HyClone SH30080.03 FetalClone® I 500ml
HyClone SH30084.03 FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN 500ml
HyClone SH30396.03 FETAL BOVINE SERUM CHARACTERIZED 500ml
HyClone SV30087.02 南美源胎牛血清 500ml
HyClone sv30087.01 南美源胎牛血清 100ml
HyClone SH30071.03 FETAL BOVINE SERUM 500ml
HyClone SH30071.02 FETAL BOVINE SERUM 100ml
HyClone SH30080.02 FetalClone® I 100ml
HyClone SH30070.02E FETAL BOVINE SERUM CHARACTERIZSED 100ml
HyClone SH30079.02 FETAL BOVINE SERUM 100ml
HyClone SH30068.02 CHARCOAL/DEXTRAN TREATED FBS,
活性炭灭活血清
100ml
HyClone SH30068.03 CHARCOAL/DEXTRAN TREATED FBS,
活性炭灭活血清
500ml
HyClone SH30066.03 FetalClone® II 500ml
HyClone SH30109.03 HyClone FetalClone® III 500ml
HyClone SH30088.03 STANDARD FETAL BOVINE SERUM 500ml
HyClone SH30088.02 STANDARD FETAL BOVINE SERUM 100ml
HyClone SH30079.03 FETAL BOVINE SERUM 500ml

 

 

第二部分  Gibco胎牛血清

 

 

品牌 货号 名称 规格
Gibco 12662-029 MSC QUALIFIED FBS 500ML 500ML
Gibco 10438-026 FBS, HI 500ML 500ML 
Gibco 10439-024 FBS, ES CELL QUAL. USDA APPD. 500ML 500ML
Gibco 10099-141 FBS, AUSTRALIAN ORIGIN 500ML 500ML 
Gibco 26400-044 FBS, DIALYZED, USA ORIGIN 500ML 500ML
Gibco 26140-079 FBS QUALIFIED, USA ORIGIN 500ML 500ML
Gibco 16141-079 FBS, ES CELL QUAL, USA ORIGIN 500ML 500ML
Gibco 16140-071 FBS, HI 500ML 500ML
Gibco 16000-044 FBS, CERT, USA ORIGIN 500ML 500ML
Gibco C2027050   FBS, URUGUAYAN ORIGIN  500ML
Gibco 12657-029 巴西胎牛 500ml
 

 

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