N2添加剂说明书Gibco（货号17502-048）_invitrogen-上海恪敏生物科技有限公司手机版

详细介绍

描述

N-2添加剂是化学成分确定的，不含血清的，基于Bottenstein’s 的N1配方的添加剂。N－2添加剂主要用于Neurobasal®培养基。

它主要被推荐用于成神经细胞瘤以及周围神经系统（PNS）与中枢神经系统（CNS）的原代细胞培养中有丝分裂后期神经元的生长和表达。N－2添加剂可以替代Bottenstein’s的N1添加剂。我们提供的N－2添加剂是100倍的浓缩体。它可用于添加了生长因子bFGF及EGF的Neurobasal®培养基，或DMEM培养基。

了解更多N2添加剂配方。.

详细说明

常用规格

Cell Type:	Neuronal Tumor Cells, Neuronal Cells
Product Size:	5 ml
Classification:	Serum-Free
Purity or Quality Grade:	Cell Culture Grade
Shipping Condition:	Dry Ice
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

引文和参考文献（2）

Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.

作者: Greenfield Jeffrey P; Leung Lawrence W; Cai Dongming; Kaasik Krista; Gross Rachel S; Rodriguez-Boulan Enrique; Greengard Paul; Xu Huaxi;
杂志: J Biol Chem （2002） 277:12128-12164
产品用途: Neuroblastoma （N2a） cells doubly transfected with human APP695 and human wild-type PS1 were maintained in medium containing 200 µg/ml G418 （or 350 µg/ml Zeocin for Rab11 expressing cell lines）. Cells were plated in equal amounts in serum-free Neurobasal media with N2 supplement, 25 mM glutamate, and 0.5 mM L-glutamine on poly-D-lysine-treated 100-mm dishes .
ID:: PN63995

Oligomeric and fibrillar species of amyloid-beta peptides differentially affect neuronal viability.

作者: Dahlgren Karie N; Manelli Arlene M; Stine W Blaine Jr; Baker Lorinda K; Krafft Grant A; LaDu Mary Jo;
杂志: J Biol Chem （2002） 277:32046-32099
产品用途: Samples were diluted in NuPage sample buffer and separated by SDS-PAGE on a 12% NuPage Bis-Tris gel （Invitrogen）. The protein was transferred to polyvinylidene difluoride membranes （Invitrogen） and then blocked for 1 h in a solution of 5% nonfat dry milk in TBS/Tween 20. Neuro-2A cells - After overnight incubation, the cells were rinsed with serum-free media containing N2 supplements （Invitrogen）
ID:: PN62678

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按细胞系排列的引文

细胞系名称	有机体类型	组织
SH-Sy5Y	Human	Brain
HT22	Mouse	Brain
Neural Stem Cells	Rat	Brain

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以下为本公司常备现货产品

*部分 invitrogen 原装产品

品牌	货号	名称	规格
invitrogen	13778-075	Lipofectamine RNAiMAX	0.75ml
invitrogen	13778-150	Lipofectamine RNAiMAX	1.5ml
invitrogen	11668-027	Lipofectamine 2000 Reagent	0.75ml
invitrogen	11668-019	Lipofectamine 2000 Reagent	1.5ml
invitrogen	15596-026	TRlzol ®Reagent	100ml
invitrogen	15596-018	TRlzol ®Reagent	200ml
invitrogen	10296-010	Trizol ® LS Reagent	100ml
invitrogen	10296-028	Trizol ® LS Reagent	200ml
invitrogen	17504-044	B-27（黄颜色）	10ml
invitrogen	12587-010	B-27（黄颜色）不含维生素A	10ml
invitrogen	17502-048	N-2 （粉红颜色）	5ml
invitrogen	18064-014	SuperScript® II Reverse Transcriptase	10,000U
invitrogen	18080-044	SuperScript® III Reverse Transcriptase	10,000U

第二部分 HyClone胎牛血清

品牌	货号	名称	规格
HyClone	SH30370.03	Australian Standard Fetal Bovine Serum	500ml
HyClone	SH30406.02	FOETAL BOVINE SERUM	500ml
HyClone	SH30401.01	New Zealand Calf Serum	500ml
HyClone	SH30066.02	FetalClone® II	100ml
HyClone	SH30088.03	STANDARD FETAL BOVINE SERUM	500ml
HyClone	SH30118.02	Newborn Bovine Calf Serum, U.S. Origin	100ml
HyClone	SH30070.02	FETAL BOVINE SERUM	100ml
HyClone	SH30087.02	COSMIC CALF® SERUM	100ml
HyClone	SH30070.03E	FETALBOVINESERUM CHARACTERIZSED	500ml
100ml	SH30070.03E	FETALBOVINESERUM CHARACTERIZSED
HyClone	SH30070.03	FETAL BOVINE SERUM	500ml
HyClone	SH30080.03	FetalClone® I	500ml
HyClone	SH30084.03	FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN	500ml
HyClone	SH30396.03	FETAL BOVINE SERUM CHARACTERIZED	500ml
HyClone	SV30087.02	南美源胎牛血清	500ml
HyClone	sv30087.01	南美源胎牛血清	100ml
HyClone	SH30071.03	FETAL BOVINE SERUM	500ml
HyClone	SH30071.02	FETAL BOVINE SERUM	100ml
HyClone	SH30080.02	FetalClone® I	100ml
HyClone	SH30070.02E	FETAL BOVINE SERUM CHARACTERIZSED	100ml
HyClone	SH30079.02	FETAL BOVINE SERUM	100ml
HyClone	SH30068.02	CHARCOAL/DEXTRAN TREATED FBS，活性炭灭活血清	100ml
HyClone	SH30068.03	CHARCOAL/DEXTRAN TREATED FBS，活性炭灭活血清	500ml
HyClone	SH30066.03	FetalClone® II	500ml
HyClone	SH30109.03	HyClone FetalClone® III	500ml
HyClone	SH30088.03	STANDARD FETAL BOVINE SERUM	500ml
HyClone	SH30088.02	STANDARD FETAL BOVINE SERUM	100ml
HyClone	SH30079.03	FETAL BOVINE SERUM	500ml

第二部分 Gibco胎牛血清

品牌	货号	名称	规格
Gibco	12662-029	MSC QUALIFIED FBS 500ML	500ML
Gibco	10438-026	FBS, HI 500ML	500ML
Gibco	10439-024	FBS, ES CELL QUAL. USDA APPD. 500ML	500ML
Gibco	10099-141	FBS, AUSTRALIAN ORIGIN 500ML	500ML
Gibco	26400-044	FBS, DIALYZED, USA ORIGIN 500ML	500ML
Gibco	26140-079	FBS QUALIFIED, USA ORIGIN 500ML	500ML
Gibco	16141-079	FBS, ES CELL QUAL, USA ORIGIN 500ML	500ML
Gibco	16140-071	FBS, HI 500ML	500ML
Gibco	16000-044	FBS, CERT, USA ORIGIN 500ML	500ML
Gibco	C2027050	FBS, URUGUAYAN ORIGIN	500ML
Gibco	12657-029	巴西胎牛	500ml

产品简介

详细介绍

描述

详细说明

常用规格

引文和参考文献（2）

Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.

Oligomeric and fibrillar species of amyloid-beta peptides differentially affect neuronal viability.

按细胞系排列的引文

常用功能直达

产品简介

详细介绍

描述

详细说明

常用规格

引文和参考文献 （2）

Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.

Oligomeric and fibrillar species of amyloid-beta peptides differentially affect neuronal viability.

按细胞系排列的引文

常用功能直达

引文和参考文献（2）