3D细胞共培养系统-3D CoSeedis，

模块化多功能3D细胞共培养系统

在3D共培养新标准

3D CoSeedis™是一种新型的无支架3D细胞共培养系统。 它由*的基于琼脂糖的基质组成，其中包含一系列圆锥形微孔。 这种布置的特殊且专有的形貌允许以高度可重复且一致的方式形成球形和非球形细胞聚集体。 阵列内的圆锥形微孔可确定聚集体的体积，从而确定细胞的生长。 此外，3D CoSeedis™系统的模块化组成允许与实际测试细胞物理分离的饲养细胞共培养（远距离共培养）。 因此，此设置负责定义条件下的协议标准化。

3D CoSeedis™矩阵的主要功能，模块化设置

与饲养细胞接触或远距离共培养
●多组合3种细胞类型以分析相互作用
●可能出现血清，血清减少或无血清的情况
●组织特定的ECM组件的应用

 基于琼脂糖的渗透性基质，允许细胞聚集体的长期3D培养

●充足的营养，氧气和分解代谢物–有效处置有毒废物
●有效利用物理分离的饲养细胞（远距离共培养）
 ●在标准或定义的媒体中进行培养

多功能适用性，适用于各种细胞
●支持球状和非球状聚集体的形成

●适用于高通量和高含量的应用

流程指导的工作流程协议
经过验证的协议●标准化和优化的组件●高度可重复的3D细胞构造●适用于高通量和高含量的应用

 适用的细胞系统我们的学术合作伙伴已成功测试了这些细胞的聚集球体形成。 在出版物中描述了详细信息：用于独立于粘附的三维细胞培养和动态体积测量的深锥形琼脂糖微孔阵列Andreas R. Thomsen等，Lab Chip，DOI 10.1039 / C7LC00832E。

3D类器官系统的标准化协议
3D CoSeedis™系统的模块化组成允许进行远程共培养，因此可以实现协议的标准化。 abc biopply的产品组合已包含适用于多种共培养的标准化协议，尤其是用于培养不同肿瘤的协议

High-Throughput / High-Content Screening Applications

3D CoSeedis™ is an ideally suited production tool to grow spheroidal 3D cell constructs in large scale, particularly for the use in HTS/HCS applications. With the various formats (2x2, 1x1, 0.5x0.5), it is possible to grow up to 1’200, 5’280, and 16’320 spheroids per plate, respectively.

The major advantages for subsequent HTS/HCS are:

• 3D CoSeedis™ matrix consists of agarose => biologically inert carrier matrix
• Substantial reduction of cell culture work (50’000 3D constructs/day ® 3 – 10  3D CoSeedis™ plates).
  > 5’000 3D constructs per 6-well plate; >16’000 per 24-well plate.
• Highly reproducible and homogeneous 3D cell aggregates across matrix and plates.
• Increased screening efficiency through highly homogenous 384-well plates seeded with 1 to several 3D constructs / well.
• Long-term studies possible: up to 70 days in culture.
• Dual and sequential treatments possible over extended
  period of time with lots of up to 900 cell aggregates.
• Fully validated for a wide range of tumour 3D aggregates.
• Modular set-up (e.g. distance co-cultures) significantly extends
  the range of testable cells in 3D including primary cells and iPS.
• Fully validated read-outs:
  • Growth monitoring
  • Viability
  • Cytotoxicity
  • Histology: paraffin and frozen
  • Colony forming assay

3D CoSeedis in High-Throughput / High-Content Screening

Research Applications

3D CoSeedis™ has been validated for various applications and according protocols have been developed. The different application notes can be downloaded here or contact us for further information.

Colony Forming Assay

A new approach to the colony forming assay in cancer research. 3D CoSeedis™ simplifies and facilitates the set-up, read-out and analysis of the assay substantially.

Download Application Note

For further details you may also refer to the following Whitepaper.

Viability / Cytotoxicity

The application note addresses viability and/or cytotoxicity of 3D cell constructs grown in 3D CoSeedis™. The protocol describes an extension to all existing 3D CoSeedis™ systems and can be implemented easily without further requirements in equipment or appliances.

Cryo-embedding

3D CoSeedis™ offers an easy and elegant way to embed 3D constructs for cryo-sectioning without the need to manipulate individual spheroids. It reduces tricky and cumbersome manipulations and prevents damage or loss of 3D constructs.

References

• Tspan8 is expressed in breast cancer and regulates E-cadherin / catenin signalling and metastasis accompanied by increased circulating extracellular vesicles
  Maren Vogelstaetter et al., J. Pathol., 2019, DOI:10.1002/path.5281

• 3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles
  Sara Rocha et al., Adv. Sci. 2018, 1800948; DOI: 10.1002/advs.201800948

• A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement
  Andreas R. Thomsen et al., Lab Chip, 2018, 18, 179; DOI: 10.1039/c7lc00832e
• Proteome Profiling of Primary Pancreatic Ductal Adenocarcinomas Undergoing Additive Chemoradiation Link ALDH1A1 to Early Local Recurrence and Chemoradiation Resistance
  V. O. Oria et al., Translational Oncology, Vol. 11, Issue 6, Dec. 2018; DOI.org/10.1016/j.tranon.2018.08.001

• A binary approach to the colony forming assay: reliable and reproducible read-outs using 3D CoSeedis™