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美国Cas9可诱导表达稳定细胞株(CRISPR适用)
美国PrimCells诱导性稳定细胞株: PrimCells建立的诱导性细胞高效开发系统,能在极短时间内筛选出低泄漏,高诱导性的稳定细胞株,成功开发出一系列核受体诱导表达的稳定细胞株产品,为多家制药公司研发部门和科研院所提供产品与服务,广受用户好评。针对客户的特定需求,PrimCells亦提供量身定制服务,通过密切的客户交流和高效的筛选体系,严格保证客户在3-4周内即获得高质量的诱导性稳定细胞株。
Introduction
CRISPR, the Clustered Regularly Interspaced Short Palindromic Repeats, is currently the most commonly used technology for genome editing. There are two distinct components in this system: a guide RNA and an RNA-guided DNA nuclease, Cas9 (CRISPR associated protein 9). When these two components are expressed together in the same cells, the genomic sequence targeted by the gRNA will be edited (modified or permanently disrupted).
Given the immediate availability of mouse and human lentiviral CRISPR gRNA libraries, CRISPR technology has tremendously benefitted the scientific community in both academia and industry. PrimCells now is providing tetracycline inducible Cas9 expressing cell lines of a variety of tissue origin to further facilitate high-throughput, CRISPR based genome-wide screening. This cell line is puromycin-, neomycin-resistant, and is also tetracycline inducible.