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华雅集团旗下授权代理TTAKARA Cellartis干细胞产品

GS2-M小鼠ES胚胎和iPS诱导多能干细胞培养基

英文名称：Cellartis GS2-M Mouse ES and iPS stem culture medium

中文名称：Cellartis GS2-M小鼠胚胎干细胞和诱导多能干细胞培养基

用途：小鼠（mouse）胚胎干细胞(ES)和诱导多能干细胞(iPS)细胞的维持培养

保存：基础培养基-20ºC；添加剂-80ºC

小鼠胚胎干细胞是*被体外分离培养并建系的干细胞，目前其相关研究已经比较完善并投入到了实际应用中，很多其他种属的干细胞研究也都会参考小鼠胚胎干细胞的研究成果。一个好的小鼠胚胎干细胞体外培养体系，需要同时满足操作简便、结果稳定、增长迅速、可重复性好、有效维持胚胎干细胞处于Ground-state等特点。

Cellartis 提供一款小鼠胚胎干细胞培养基GS2‐M，其成分确定、无血清，支持无饲养层培养，通过添加两种特别的小分子抑制剂（CHIR99021，PD0325901=2i）来阻断分化信号通路GSK3β和 ERK/MEK，进而维持小鼠胚胎干细胞处于Ground state。值得一提的是，使用GS2-M培养基培养的小鼠胚胎干细胞，与添加血清和LIF的培养基培养相比，可以更好地维持小鼠胚胎干细胞处于Ground-state。

GS2-M与细胞因子leukemia inhibitory factor (LIF)一同使用时，可以通过调控Nanog信号通路实现将partial or pre‐iPS cells转化成为fully pluripotent iPS cells(1, 2)。

GS2‐M is a compley defined, serum‐free cell culture media formulation for the generation and long-term maintenance of human and mouse embryonic stem (ES) and induced pluripotent stem (iPS) cell lines. The medium contains two selective small molecule inhibitors that block differentiation‐inducing signals and promote cell survival.

GS2-M medium can be used in combination with the self-renewal cytokine leukemia inhibitory factor (LIF) to convert partial or pre‐iPS cells from mouse into fully pluripotent iPS cells via upregulation of Nanog (1, 2).

产品特点：

Compley defined, serum-free formulation

Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK and maintain ES and iPS cells in a pluripotent state

Allows long-term maintenance of germline-competent mouse and human ES and iPS cells

产品应用

小鼠部分或pre-iPS细胞转化为*多能干细胞*

将人ES细胞或iPS细胞转化为初始(naïve)干细胞*

维持纯ES和iPS细胞的多能状态

*在Forskolin和/或LIF存在下

Conversion of partial or pre-iPS cells from mouse into fully pluripotent stem cells*

Conversion of human pre-ES or iPS cells into naïve stem cells*

Maintenance of pure ES and iPS cells in a pluripotent state

*In the presence of Forskolin and/or LIF

组份：

100 ml medium

100 µl 2i inhibitor supplement

自备试剂：

A. For mouse ES and iPS cells.

Culture vessels are pre-coated with either 0.1% gelatin type A in PBS solution (at room temperature for 15 minutes or more), or precoated sequentially with 0.01% poly-L-ornithine hydrobromide (at 37℃ for 30 minutes or more), washing twice with PBS, followed by 10 μg/mL laminin in PBS (at 37℃ for 3 hours or more).

B. For human iPS cells.

Please refer to Wang W, et al. (2011) reference for more detailed reagent and culture requirements to generate and propagate ‘naïve’ human iPS cells4.

A.对于小鼠ES和iPS细胞。

培养容器在PBS溶液（室温下15分钟或更长时间）中预先用0.1％明胶A型包被，或者预先用0.01％聚-L-鸟氨酸氢溴酸盐（在37℃下30分钟或更多）预包被， 用PBS洗涤2次，然后用PBS中的10μg/ mL层粘连蛋白（37℃下3小时以上）洗涤。

B.对于人类iPS细胞。

请参考王伟等人（2011）更详细的试剂和培养要求以生成和传播“幼稚”人类iPS细胞的参考文献4。

保存：Upon receipt, store the media at -20℃ and the GS2-M 2i inhibitor supplement at -80℃ until ready to use. When stored under these conditions, the products are stable for 12 months from the date of manufacture (see label).Once thawed and combined, store at 4℃ and use within 2 weeks.This product is light sensitive, and should be protected from light.

参考文献：

1. Silva J, et al. (2008) Promotion of reprogramming to ground state pluripo-tency by signal inhibition. PLoS Biol. 6: e253.

2. Silva J, et al. (2009) Nanog is the gateway to the pluripotent ground state.Cell. 138(4): 722-737.

3. Nichols J, et al. (2009) Validated germline-competent embryonic stem cell lines from non-obese diabetic mice. Nat Med. 15: 814-818.

4. Wang W, et al. (2011) Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog. PNAS (USA). 108(45): 18283-18288.

5. Reviewed in: Malaver-Ortega LF, et al. (2012) Theriogenology. 78(8): 1749-1762.

Cellartis 小鼠/大鼠胚胎干细胞培养基系列：成分确定、无血清培养基

GS2-M® 小鼠胚胎干细胞和多能诱导干细胞培养基，无饲养层，不依赖LIF

iSTEM™ 小鼠胚胎干细胞培养基，难获得ES细胞小鼠

GS1-R® 大鼠多能干细胞培养基，需要饲养层，胚胎干细胞和胚胎生殖细胞（EG）

​华雅集团旗下授权代理TTAKARA Cellartis干细胞产品，Cellartis的多能细胞培养系统包括iPS诱导多能干细胞株、iPS诱导多能干细胞和ES胚胎干细胞培养基，iPS基因编辑用单细胞克隆培养基和多能细胞检测用单克隆抗体。

重庆市华雅干细胞技术有限公司以“专注干细胞研究，提升干细胞品质”为发展理念，专业从事干细胞基础研究、干细胞再生医学临床应用研究及干细胞技术服务，自主研发产品热卖中：人间充质干细胞无血清培养基 

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