DMEM(H)(含双抗，不含丙酮酸钠) 培养基
货号：12100-046
规格：10*1L
英文名称：DMEM, Powder, High Glucose
储存条件：2-8℃
单位：瓶

描述

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

内容及储存

储存条件：2-8° C
运输条件：环境温度
保存期：自生产日期起36个月

DMEM(H)(含双抗，不含丙酮酸钠) 培养基

 参考文献：

《Effects of C-myc gene silencing on interleukin-1β-induced rat chondrocyte cell proliferation, apoptosis and cytokine expression》 作者:Jian Zou，Xiao-Lin Li，Zhong-Min Shi，Jian-Feng Xue 期刊:Journal of Bone and Mineral Metabolism 影响因子:2.219 PMID:28616752
《Hematopoietic Substrate-1-Associated Protein X-1 Regulates the Proliferation and Apoptosis of Endothelial Progenitor Cells Through Akt Pathway Modulation》 作者:XIN-BIN GUO,XIN DENG,YING WEI 期刊:Wiley Online Library 影响因子: PMID:29139175
《Molecular characterization of Cyprinid herpesvirus 3 encoded viral interleukin10》 作者:Ping Ouyang，Ruixue Yang，Lizi Yin，Yi Geng，Weiming Lai，Xiaoli Huang，Defang Chen，Jing Fang，Zhengli Chen，Li Tang，Min He，Chao Huang，Wentao Liu，Kaiyu Wang 期刊:Fish & Shellfish Immunology 影响因子:3.298 PMID:30926478
《Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A》 作者:Lingjuan Du, Guojian Li, Yong Yang, Guokai Yang, Jia Wan, Zhenhuan Ma, Yi Hou 期刊:International Journal of Clinical and Experimental Pathology 影响因子:0.205 PMID:
《Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway》 作者:Panpan Zhang,Lingyue Hua,Huan Hou, Xingyue Du,Zhiqiang He, Menghan Liu, Xiaojuan Hu, Nianlong Yan 期刊:International Journal of Molecular Medicine 影响因子:2.928 PMID:30272329
《Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes》 作者:Lixin Li,Juan Wang,Yun Bai,Jingwei Li,Xiuju Yu,Xiaomao Luo,Zhiwei Zhu,Xiaoyan He,Yanjun Dong,Hongquan Li,Haidong Wang 期刊:Acta Histochemica 影响因子:4.239 PMID:31047685
《Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR‐101‐3p/WEE1 axis》 作者:Xin Chen, Nuobei Zhang 期刊:Cell Biology International 影响因子:2.127 PMID:30488993
《Knockdown of USP28 enhances the radiosensitivity of esophageal cancer cells via the c‐Myc/hypoxia‐inducible factor‐1 alpha pathway》 作者:Zhang Weili， Liu Zhikun，Wang Jianmin， Tian Qingbao 期刊:Journal of Cellular Biochemistry 影响因子:3.448 PMID:30206969
《HDAC2, but not HDAC1, regulates Kv1.2 expression to mediate neuropathic pain in CCI rats》 作者:Zhihua Li, Yanyan Guo, Xiuhua Ren, Lina Rong, Minjie Huang, Jing Cao, Weidong Zang 期刊:Neuroscience 影响因子:3.244 PMID:31022463
《Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture》 作者:Yi-Meng Duan, Ying Jin, Meng-Li Guo, Leng-Xin Duan, and Jian-Gang Wang 期刊:Journal of Cancer 影响因子:3.182 PMID:30087713
《青蒿琥酯对高糖条件下血管内皮细胞ICAM-1和MMP-9表达的影响》 作者:葛朋飞,姜 涛,宗 瑶,杨雪娇,马玉娜,王云霄,孙 哲,曹子群 期刊:Hans Journal of Surgery 影响因子: PMID:
《Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor》 作者:QingqingHu1NaYao1JieWuMingyiLiuFanglanLiuHongZhangYuqingXiongChunhuaXia 期刊:Biochemical Pharmacology 影响因子:4.235 PMID:30414935
《Characterization of novel exopolysaccharide of Enterococcus faecium WEFA23 from infant and demonstration of its in vitro biological properties》 作者:Kaiying Jia,Xueying Tao,Zhengqi Liu,Hui Zhan,Weijun He,Zhihong Zhang,Zheling Zeng,Hua Wei 期刊:International Journal of Biological Macromolecules 影响因子:3.909 PMID:30594615
《Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway》 作者:Zongfeng Zhang, Lina Wang ,Juan Du ,Yuanbo Li, Huilun Yang, Chenxi Li, Hui Li, Haiyang Hu 期刊:Oncology Letters 影响因子:1.664 PMID:28101233
《MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3》 作者:Shiming Yang, Zhi Chen, Daguang Fan, Rui Zhang, Yuhong Zhang and Shusheng Wu 期刊:RSC Adv. 影响因子:2.789 PMID:
《A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein》 作者:Kangkang Guo, Lei Xu, Mengmeng Wu, Yufeng Hou, Yanfen Jiang, Jiangman Lv, Panpan Xu, Zhixin Fan, Ruiqi Zhang, Fushan Xing and Yanming Zhang 期刊:Frontiers in Microbiology 影响因子:4.019 PMID:30671053
《Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro》 作者:Xinmou Wu，Minqing Li，Zhao Yang，Min Su，Bin Yang 期刊:Environmental Science and Pollution Research 影响因子:2.8 PMID:28887612
《Trophoblast glycoprotein promotes pancreatic ductal adenocarcinoma cell metastasis through Wnt/planar cell polarity signaling》 作者:Ping He, Shuheng Jiang, Mingze Ma ,Yang Wang, Rongkun Li, Fang Fang, Guangang Tian ,Zhigang Zhang 期刊:Molecular Medicine Reports 影响因子:1.922 PMID:25738465
《High Expression of IL-4R Enhances Proliferation and Invasion of Hepatocellular Carcinoma Cells》 作者:C Guo, Y Ouyang, J Cai, L Xiong 期刊:The International Journal of Biological Markers 影响因子:1.449 PMID:28665449
《Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro》 作者:C Xu, C Liu, W Huang, S Tu, F Wan 期刊:Acta Biochim Biophys Sin 影响因子:2.224 PMID:23419720
《TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression》 作者:L Sun, Z Dong, H Gu, Z Guo, Z Yu 期刊:Cancer Management and Research 影响因子:3.702 PMID:30697069
《Selective anticancer activity of the novel steroidal dihydropyridine spirooxindoles against human esophageal EC109 cells》 作者:Xiaoli Shi,Tiantian Qin,Weihua Liu,Xueyan Zhang,Leilei Li,Junfeng Huo,Kairui Zhou,Dongxiao Yang,Yanling Zhang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影响因子:2.068 PMID:
《Gastroprotective effect of gamma-aminobutyric acid against ethanol-induced gastric mucosal injury》 作者:Min Xie,Haihong Chen,Shaoping Nie,Wei Tong,Junyi Yin,Mingyong Xie 期刊:Chemico-Biological Interactions 影响因子:3.296 PMID:28456544
《共轭亚油酸异构体对胰岛素抵抗脂肪细胞消耗葡萄糖的影响》 作者:刘晓华， 刘萍， 李海星， 李超波， 曹郁生 期刊:南昌大学学报(理科版), 影响因子:1.187 PMID:
《蜂胶中 ４ 种黄酮对 ＨｅｐＧ２ 细胞胰岛素抵抗的改善作用》 作者:梁霞丽　赵亚周　张红城　彭文君 期刊:中国农业科技导报 影响因子:1.439 PMID:
《Upregulated lncRNA small nucleolar RNA host gene 1 promotes 1‐methyl‐4‐phenylpyridinium ion‐induced cytotoxicity and reactive oxygen species production through miR‐15b‐5p/GSK3β axis in human dopaminergic SH‐SY5Y cells》 作者:Na Xie， Jinxing Qi， Shuang Li， Jianzhong Deng， Yuan Chen， Yajun Lian 期刊:Journal of Cellular Biochemistry 影响因子:3.448 PMID:30302821
《c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma》 作者:Shangfeng Li, Shengkai Zhang & Jie Chen 期刊:Cancer Gene Therapy 影响因子:4.681 PMID:30607006
《Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map》 作者:Jiayu Gao, Xiao Yang, Jiangxia Hu and Weiping Yin 期刊:International Journal of Pharmacology 影响因子:0.754 PMID:
《Label?free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion》 作者:Lili Zhao, Meng Zhao, Yu Yang, Yajun Gu ,Fang Zheng ,Xuan Wang ,Zhiyuan Zheng, Xuguo Sun 期刊:Oncology Letters 影响因子:1.871 PMID:30944642
《Emodin Attenuates Lipopolysaccharide-Induced Injury via Down-Regulation of miR-223 in H9c2 Cells》 作者:Yuping Yang, Zijun Jiang, Dong Zhuge 期刊:International Heart Journal 影响因子:2.226 PMID:30745529
《miR?9 depletion suppresses the proliferation of osteosarcoma cells by targeting p16》 作者:Song Gao, Jianchao Wang, Shujian Tian, Jianping Luo 期刊:International Journal of Oncology 影响因子:3.571 PMID:31081054
《Long Non-Coding RNA NIFK-AS1 Augments the Oncogenicity of Hepatocellular Carcinoma by Regulating miR-497/BIRC5 Axis》 作者:Yajing Feng,Lu Sun,Yajuan Feng,Zhixian Guo 期刊:SSRN 影响因子: PMID:
《Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress》 作者:Lixin Huang,Min Huang,Mingyue Shen,Pingwei Wen,Ting Wu,Yuzhen Hong,Qiang Yu,Yi Chen,Jianhua Xie 期刊:International Journal of Biological Macromolecules 影响因子:4.784 PMID:31254574
《miR-185 silencing promotes the progression of atherosclerosis via targeting stromal interaction molecule 1》 作者:Ming Fang, Yanfei Li, Yingbiao Wu, Zhongping Ning, Xuejun Wang & Xinming Li 期刊:Cell Cycle 影响因子:3.259 PMID:30784343
《Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA》 作者:Shan Huang , Zheng Wu , Yunyun Cheng , Wenzhen Wei  Linlin Hao 期刊:Acta Biochimica et Biophysica Sinica 影响因子:2.502 PMID:31089713
《Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression》 作者:Longfei Xiao ， Liangli Song，  Junjie Hu ， Quanwei Zhang ， Yuting Jiang ， Hongwei Duan ， Yong Zhang， Xingxu Zhao ， Yongli Hua 期刊:Journal of Cellular Physiology 影响因子:4.522 PMID:30671954
《Biological characteristics of heat shock protein 90 in human liver cancer cells》 作者:Li Qin, Haixin Huang, Jian Huang, Guodong Wang, Jinxin Huang, Xintian Wu, Jinzhuan Li, Weili Yi, Longjing Liu, and Dongning Huang 期刊:American Journal of Translational Research 影响因子:3.266 PMID:31105855
《Effect of Artesunate on the Expression of ICAM-1 and MMP-9 in Vascular Endothelial Cells under High Glucose Condition》 作者:Pengfei Ge, Tao Jiang, Yao Zong, Xuejiao Yang, Yuna Ma, Yunxiao Wang, Zhe Sun, Ziqun Cao 期刊:Hans Journal of Ophthalmology 影响因子:1.58 PMID: