Anti-Human CD28 SAFIRE Purified   CD28单抗

产品英文名称：Anti-Human CD28 SAFIRE Purified

产品中文名称：CD28单克隆抗体/抗人CD28抗体

BioGems CD28单抗特点

极低的内毒素含量，低于0.01EU/μg蛋白 

不含叠氮钠，适用于体内或体外实验

高蛋白纯度

应用 BioGems CD28单抗适用细胞：T细胞

作用机理 CD28单克隆抗体特异性地与人类44 kDa同源二聚体跨膜糖蛋白CD28结合，CD28在大多数成熟的T淋巴细胞、浆细胞和胸腺细胞的表面表达。CD28是B7-1(CD80)和B7-2(CD86)的配体，T淋巴细胞的共刺激分子，并能增强T和B淋巴细胞间的相互作用。据报道，T淋巴细胞刺激产生IL-2依赖于单克隆抗体,这表明CD28分子呈现出具有不同功能的亚区域。CD28单克隆抗体诱导Ca2+流入Jurkat T淋巴细胞。其他研究表明CD28与信号转导有关。

Description

The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.

质量控制

BioGems CD28单克隆抗体经过亲和层析纯化。

内毒素水平：蛋白质小于0.01 EU/µg，鲎试剂法检测。

订购信息        

红荣微再代理的BioGems CD3/CD28单抗正进行年末*活动：买二送一（四折之后），并赠送抗霾礼盒

Biogems是Peprotech的抗体品牌。CD3/CD28单抗BioGems*现正进行4折*。

CD3  500ug 现价4130元 *(4折) 1652元

CD28  500ug 现价3640元 *(4折) 1456元

BioGems的SAFIRE (Sodium Azide Free Immensely Reduced Endotoxins) 类产品是BioGems品牌下的功能性抗体产品，经特殊工艺生产、纯化，具有诸多特点，可用于免疫细胞功能的激活、阻断、中和研究，是肿瘤免疫研究的重要工具。

SAFIRE纯化CD3单抗 (clone: OKT3)可以与TCR复合物结合，激活T细胞；SAFIRE纯化CD28单抗 (clone: CD28.2)是 T细胞激活、增殖的辅助刺激分子。

红荣微再*

CD3 CD28单抗买二送一！

在4折基础上再加优惠。

买二：CD3、CD28任选两支。

送一：CD3、CD28任选一支。

赠送抗霾礼盒

抗霾礼盒

易可滤挂机空调伴侣防PM2.5空调过滤

易可滤防雾霾过滤PM2.5可更换式口罩

价值600多元

活动时间：2017年12月15日 - 2018年3月31日

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员

CD3/CD28功能性抗体应用：CAR-T及免疫细胞研究

CAR-T介绍

       通过增强免疫系统对肿瘤的反应来治疗癌症的肿瘤免疫疗法被认为具有巨大的临床应用前景。其中的过继性免疫疗法—CAR-T疗效 法，正受到科研与临床的极大关注，已有多家研究机构 (如Baylor, Fred Hutchinson, NCI)展开临床研究，而今年FDA更是已经批准了诺华和Kite两款CAR-T治疗药物。

Anti-Human CD28 SAFIRE Purified 说明书

Catalogue Number : 10311-25

RUO: For Research Use Only. Not for use in diagnostic procedures.

Product Information

Clone: CD28.2

Format/Conjugate: SAFIRE Purified

Concentration: 2 mg/mL

Reactivity: Human

Laser: Not Applicable

Peak Emission: Not Applicable

Peak Excitation: Not Applicable

Filter: Not Applicable

Brightness (1=dim,5=brightest): Not Applicable

Isotype: Mouse IgG1, kappa

Formulation: Phosphate-buffered aqueous solution, ph7.2.

Storage:Product should be kept at 2-8°C and protected from prolonged exposure to light.

Applications: FC, FA, IHC, IP

Description

The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28,expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80)and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.

Preparation & Storage

The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity

chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Application Notes

The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. It is recommended that the reagent be titrated for optimal performance for each application.

References

1.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

2.Nunès, J., Klasen, S., Ragueneau, M., Pavon, C., Couez, D., Mawas, C., ... ; Olive, D. (1993). CD28 mAbs with distinct binding properties differ in

their ability to induce T cell activation: analysis of early and late activation events.International immunology,;5(3), 311-315.

3.Karlsson, I., Malleret, B., Brochard, P., Delache, B., Calvo, J., Le Grand, R., ; Vaslin, B. (2007). FoxP3+ CD25+ CD8+ T-cell induction during primary

simian immunodeficiency virus infection in cynomolgus macaques correlates with low CD4+ T-cell activation and high viral load.;Journal of virology,;81(24),

13444-13455.

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员