违禁品MET酶联免疫试纸

广州健仑生物科技有限公司

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保质期：2年

MET胶体金抗原检验检测试纸（进口）

MET胶体金抗原检验检测试纸（进口）

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【公司名称】 广州健仑生物科技有限公司

【 市场部 】       杨永汉
【】 
【腾讯  】
【公司地址】 广州市清华科技园健新基地番禺石楼镇健启路63号二期2幢101-103室

家兔肺泡巨噬细胞的分离和纯化巨噬 细胞的分离和纯化1、取2～3公斤重的家兔，耳缘静脉注射10ml空 气处死动物或注射2ml（含130mg）戊巴比妥麻醉动物。仰卧固定 ，消毒，剪开胸壁。以下过程注意无菌操作。小心从环状软骨下 方剪下气管，分离心脏和血管，取出肺，剪去脂肪和结缔组织。 用无菌纱布小心擦净肺表面，称重。2、分离肺泡巨噬细胞：用夹 子夹住气管，使肺悬空。向气管中注射40ml无菌的冷生理盐水， 让生理盐水进入全部肺泡。用镊子提起气管，从夹子下面剪断气 管，将肺中的液体倒入离心管中。再用同样方法，用40ml无菌冷 生理盐水冲洗肺泡，合并浸出液，置4℃。3、分离肺组织中的巨 噬细胞：取出肺泡巨噬细胞后，剪去气管，将肺组织放在有冷 RPMI-1640培养液的平皿中。平皿置冰上，用吸满冷RPMI-1640培 养液的20ml注射器接23号针头，一边灌注一边用针头梳离肺组织 ，直到肺组织与支气管树全部分离。使肺组织通过00目的钢丝网 ，分离单细胞。4、以下过程均在4℃中进行。
Isolation and Purification of Alveolar Macrophages from Rabbits Macrophage Isolation and Purification 1. Rabbits weighing 2 to 3 kilograms were killed by intravenous injection of 10 ml of air into the ear vein or injected with 2 ml (containing 130 mg) of pentobarbital anesthetized animals. . Supine fixation, disinfection, cut the chest wall. The following procedure pays attention to aseptic operation. Carefully cut the trachea from beneath the cricoid cartilage, separate the heart and blood vessels, remove the lungs, and cut fat and connective tissue. The lung surface was carefully wiped with sterile gauze and weighed. 2. Detach the alveolar macrophage: clip the trachea with the clip and let the lungs float. Into the trachea, 40 ml of sterile cold saline was injected, and physiological saline was allowed to enter all alveoli. Pull the trachea with forceps, cut the trachea from underneath the clamp, and pour the lung fluid into the centrifuge tube. In the same manner, the alveoli were washed with 40 ml of sterile saline, and the combined leachate was set at 4°C. 3. To isolate macrophages from lung tissue: After removing the alveolar macrophages, cut the trachea and place the lung tissue in a plate with cold RPMI-1640 medium. The dish was placed on ice, and a 23-gauge needle was connected to a 20-ml syringe that was filled with cold RPMI-1640 medium, and the needle was used to perfuse the lung tissue until the lung tissues were compley separated from the bronchial tree. The lung tissue was passed through a 00 mesh wire mesh to separate single cells. 4. The following procedures were all performed at 4°C..