单抗144734-HRP中英文实验说明

2019年3月，苏州蚂蚁淘生物科技有限公司正式获得USBIo中国大陆区总代理授权。USBIo全称United States BIOLOGical，也简称为USBIological。位于麻省斯万普斯科特(Swampscott, MA)。是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。

主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。

uSBIo品牌单抗144734-HRP实验说明

IL-17（白细胞介素-17），初由Rouvier等人鉴定为来自啮齿动物T细胞杂交瘤的转录物。 1993年，是一组称为IL-17家族的细胞因子的创始成员。在啮齿动物中称为CTLA8，IL-17显示出与由T-淋巴细菌性rhADInovirus疱疹病毒saimiri的开放阅读框编码的病毒IL-17高度同源。小鼠IL-17与大鼠具有85％的氨基酸序列同源性。白细胞介素17是一种细胞因子，通过增加各种组织中的趋化因子产生，将单核细胞和嗜中性粒细胞募集到炎症部位，作为延迟型反应的有效介质，类似于干扰素γ。 IL-17由T辅助细胞产生并由IL-23诱导，其导致延迟型反应中的破坏性组织损伤。白细胞介素17作为一个家族起着促炎细胞因子的作用，它可以通过细胞外病原体对免疫系统的侵袭作出反应，并诱导病原体细胞基质的破坏。白细胞介素17与肿瘤坏死因子和白细胞介素-1协同作用。为了引发其功能，IL-17结合称为IL-17R的I型细胞表面受体，其中存在至少三种变体IL17RA，IL17RB和IL17RC。

单抗144734-HRP中英文实验说明

IL-17(Interleukin-17), originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et al. in 1993, is the founding member of a group of cytokines called the IL-17 family. Known as CTLA8 in rodents, IL-17 shows high homology to viral IL-17 encoded by an open reADIng frame of the T-lymphotropic rhADInovirus Herpesvirus saimiri. Mouse IL-17 shares 85% amino acid sequence homology with rat. Interleukin 17 is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation, similar to Interferon gamma. IL-17 is produced by T-helper cells and is induced by IL–23 which results in destructive tissue damage in delayed-type reactions. Interleukin 17 as a family functions as a proinflammatory cytokine that responds to the invasion of the immune system by extracellular pathogens and induces destruction of the pathogen’s cellular matrix. Interleukin 17 acts synergistically with tumor necrosis factor and interleukin-1. To elicit its functions, IL-17 binds to a type I cell surface receptor called IL-17R of which there are at least three variants IL17RA, IL17RB, and IL17RC.

UniProt Number

Q62386

Gene ID

IL17A

Applications

Suitable for use in ELISA, Western Blot.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Storage and Handling

Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Immunogen

E. coli-derived mouse IL-17 recombinant protein (Position: A26-A158).

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horserADIsh peroxidase (HRP).

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes mouse IL-17/IL-17A. No crossreactivity with other proteins.