产品描述

一瓶封闭缓冲液，500毫升。所有western印迹只需要5分钟的封闭

每个印迹-5分钟每个印迹阻断

五分钟的阻断缓冲液，适用于所有印迹。

EveryBlot阻断缓冲液为所有蛋白质印迹提供5分钟的阻断和较大灵敏度，无论检测方法如何。

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功能和优点

减少非特异性抗体结合以减少背景，同时保持优异的敏感性

用于化学发光和荧光检测

不含磷酸盐缓冲液。非常适合与磷酸特异性抗体一起使用

5分钟内完成封闭

Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots

competitor-everyblot-5 minute everyblot blocking

A five minute blocking buffer for ALL western blots.

EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.

Features and Benefits

Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity

For both chemiluminescent and fluorescent detection

Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies

Complete blocking in 5 minutes

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使用建议

   对于小型印迹，使用至少10 ml用于阻断和抗体孵育步骤。对于中等大小的印迹，至少使用20毫升。

将块加入膜中，搅拌孵育5分钟

将第一抗体和第二抗体稀释在全强度块中，并在搅拌下孵育1小时

对于PVDF的荧光检测，将SDS添加到第二抗体溶液中的0.02%。

   Recommended Protocol

For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.

Add block to the membrane and incubate for 5 minutes with agitation

Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation

For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.