Normal Human Glomerular Endothelial Cells

人肾小球内皮细胞

Normal Human Glomerular Endothelial Cells (NhGEC) are specialized microvascular cells that make up the innermost layer of the glomerular filtration barrier. They are separated from podocytes by a thin layer of glomerular basement membrane. These cells exhibit cobblestone morphology at confluence and present fenestrations specific of this cell type.

The NhGEC are obtained after cortex digestion, and single glomerular isolation. Glomeruli are further digested and then NhGEC are isolated by CD31 positive selection as a pure population.

The NhGEC express factor VIII-related antigen, angiotensin converting enzyme activity, and endocytosed acetylated low-density lipoproteins. They are also characterized by the presence of intracellular Weibel-Palade bodies and caveolae and microvillous projections on the luminal surface.

The NhGEC will be evaluated for the expression of endothelial specific markers (e.g. CD31, vWF and Ve-Cadherin) by  immunofluorescence.

NhGEC can be expanded between 5 to 8 passages, while maintaining their particular characteristics.

NhGEC are terminally differentiated cells and present no significant differentiation capacity.

The NhGEC are for research use only and not approved for human use, or for in vitro diagnostic testing.

Normal Human Glomerular Endothelial Cells

人肾小球内皮细胞

PBMC分离试剂盒

人外周血单个核细胞分离试剂盒PBMC分离液

人PBMC分离试剂盒 人PBMC分离液

猴外周血单个核细胞分离试剂盒PBMC分离液

猴PBMC分离试剂盒 猴PBMC分离液

比格犬外周血单个核细胞分离试剂盒PBMC分离液

比格犬PBMC分离试剂盒 比格犬PBMC分离液

狗外周血单个核细胞分离试剂盒PBMC分离液

狗PBMC分离试剂盒 狗PBMC分离液

大鼠外周血单个核细胞分离试剂盒PBMC分离液

大鼠PBMC分离试剂盒 大鼠PBMC分离液

小鼠外周血单个核细胞分离试剂盒PBMC分离液

小鼠PBMC分离试剂盒 小鼠PBMC分离液

兔外周血单个核细胞分离试剂盒PBMC分离液

兔PBMC分离试剂盒 兔PBMC分离液

猪外周血单个核细胞分离试剂盒PBMC分离液

猪PBMC分离试剂盒 猪PBMC分离液

豚鼠外周血单个核细胞分离试剂盒PBMC分离液

豚鼠PBMC分离试剂盒 豚鼠PBMC分离液

骨髓单个核细胞分离试剂盒Bone Marrow Mononuclear Cells Isolation Kit

脾单个核细胞分离试剂盒 Spleen Mononuclear Cells Isolation Kit

红细胞分离试剂盒

人红细胞分离试剂盒 红细胞分离液

猴红细胞分离试剂盒 红细胞分离液

比格犬红细胞分离试剂盒 红细胞分离液

大鼠红细胞分离试剂盒 红细胞分离液

小鼠红细胞分离试剂盒 红细胞分离液

ADME相关产品

Ⅰ相代谢稳定性试剂盒

Ⅱ相代谢稳定性试剂盒

CYP450 酶代谢表型研究试剂盒（化学抑制法/7种抑制剂）

CYP450 酶代谢表型研究试剂盒（重组酶法/7种酶）

CYP450 酶代谢表型研究试剂盒（重组酶法/单酶）

酶抑制（IC50）研究试剂盒（7种特异性底物）

酶抑制（IC50）研究试剂盒（单个酶）

NADPH再生系统

UGT孵育系统

0.1M PBS

肝微粒体（人，猴，犬，大鼠，小鼠）

肝S9（人，猴，犬，大鼠，小鼠）

肝原代细胞（人，猴，犬，大鼠，小鼠）

CYP450（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

UGT酶

探针底物，代谢产物，抑制剂（CYP1A2，CYP2A6，CYP2B6，CYP2C8，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

ADME服务项目

肝微粒体代谢稳定性（人，猴，犬，大鼠，小鼠）

肝细胞代谢稳定性试验（人，猴，犬，大鼠，小鼠）

代谢表型研究

代谢产物鉴定

代谢途径鉴定

种属比较研究

CYPP450抑制实验（CYP1A2，CYP2A6，CYP2C9，CYP2C19，CYP2D6，CYP2E1，CYP3A4）

CYPP450诱导实验

血浆蛋白结合率测定

血浆稳定性试验

跨膜转运试验

药物-药物相互作用

毒理学研究

Innovative Reagents for Innovative Research

汇智和源致力于为创新药企业及生命科学研究机构提供高品质的生物试剂。