PS Bioscience的PARP/PARPTrap™筛选和检测服务

PS Bioscience的PARP/PARPTrap™筛选和检测服务

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2023-12-19 23:23:59
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BPS Bioscience Inc.

BPS Bioscience Inc.

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提供商:BPSBioscience服务名称:PSBioscience的PARP/PARPTrap™筛选和检测服务PARP(PolyADP-RibosePolymerase)proteinsarealargefamilyof17membersthatcatalyzetheADP-ribosylationofproteinsand

详细介绍

PARP (Poly ADP-Ribose Polymerase) proteins are a large family of 17 members that catalyze the ADP-ribosylation of proteins and, for PARP1 and PARP2, of DNA. PARPs are part of a network of 150 proteins involved in the DNA damage response, which constantly scan and repair DNA to maintain genome integrity. The PARP proteins are involved in a wide range of biological functions: DNA repair, chromatin remodeling, mitotic spindle assembly, regulation of RNA turnover, regulation of gene expression, apoptosis, and more.

Accelerate your drug discovery and development projects using PARP or PARPtrap™ screening services. We offer the largest PARP proteins panel on the market, allowing you to compare efficacy across our entire PARP family.

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Our team of experts, using our in-house developed biochemical assay kits, will:

  • Screen for PARP inhibitors from large compound libraries, measured by ELISA, AlphaLISA®, or Fluorescence Polarization assays

  • Screen for PARG inhibitors

  • Compare potency with IC50 screens

  • Answer your questions and guide your project in a timely manner

  • Deliver detailed results promptly and on time

Enzymatic Activity by ELISA

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ELISA: Histone proteins are coated on a plate. Biotin-labeled NAD+ is added with the PARP enzyme. PARP-mediated ribosylation activity is detected by adding Streptavidin-HRP (horseradish peroxidase), which binds to the newly formed ribosylation branch, and a chemiluminescent or colorimetric HRP substrate.

Enzymatic Activity by AlphaLISA®

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AlphaLISA® Assay: a PARP enzyme is incubated with biotinylated histone substrate and NAD+ for an hour. An ADP-ribose binding reagent is added with an acceptor bead, then a streptavidin-conjugated donor bead is added. Excitation of the donor bead results in excitation of the acceptor bead and light emission. This is a homogeneous assay.

PARP Trapping

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Fluorescence Polarization Assay: The assay uses fluorescent DNA probes that emit differently polarized light depending on PARP binding. Addition of a PARP inhibitor prevents ribosylation and results in the trapping of PARP onto the fluorescent oligonucleotide duplex, which increases the Fluorescence Polarization signal in a dose dependent manner. This is a homogeneous assay.

Molecular Degradation Optimization

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AlphaLISA® Assay: A degrader of interest interacts with both PARP and CRBN, bringing them in proximity. PARP-GST is recognized by the GSH donor bead, while CRBN-FLAG binds to anti-FLAG conjugated to the acceptor bead. Excitation of the donor bead results in excitation of the acceptor bead and light emission. This is a homogeneous assay.

Available Service Assays

Chemiluminescent Assays*:

PARGPARP1PARP2PARP3PARP4PARP5A (TNKS1)PARP5B (TNKS2)PARP6
PARP7PARP8PARP10PARP11PARP12PARP14PARP15PARP16
*NOTE: As of April 2022, these protocols have been re-optimized for performance. Previous versions of this protocol are available upon request.


Colorimetric Assays:

PARP1PARP2PARP5A (TNKS1)PARP5B (TNKS2)

Homogenous Assays:

PARP1PARP2PARP3PARP5A (TNKS1)PARP5B (TNKS2)PARP11

PARPTrap™ Assays:

PARP1Trap PARP2Trap

如果您需要了解更多关于我们的服务的信息,请联系info。

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