论文信息：
论文题目： AMFR drives allergic asthma development by promoting alveolar macrophage–derived GM-CSF production
期刊名称：JEM- J Exp Med
时间期卷：(2021) 218 (5): e20201452.
在线时间：2021年3月3日
DOI： doi.org/10.1084/jem.20201452
Liposoma巨噬细胞清除剂氯膦酸盐脂质体Clodronate Liposomes见刊于JEM：
Liposoma巨噬细胞清除剂氯膦酸盐脂质体Clodronate Liposomes的材料和方法：
JEM期刊率膦酸盐脂质体清除巨噬细胞专业论文：巨噬细胞清除解决方案。
Depletion of monocytes/macrophages and granulocytes
For depletion of granulocytes, mice were injected i.p. with 200 µg anti-Ly6G antibody or control antibody (Bio X Cell) 3 d before the first vincristine administration and then together with vincristine (i.p.; 0.5 mg/kg) injection as described previously (Daley et al., 2008; Starobova et al., 2019a). For depletion of monocytes, clodronate liposomes (10 µl/g of a 5-mg/ml solution) or control liposome-encapsulated PBS (Liposoma Research) were injected i.p. 3 d before the first vincristine administration and then together with vincristine (i.p.; 0.5 mg/kg) injection as described previously (Barclay et al., 2007; Old et al., 2014; Starobova et al., 2019a; Van Rooijen and Sanders, 1994). To confirm depletion of monocytes or granulocytes, the spleen or blood were collected after two doses of clodronate or Ly6G and/or 24 h after first vincristine (i.p.; 0.5 mg/kg) injection.
