脑啡肽抗体说明书

Rabbit Anti-Enkephalin/P-ENK

 Quantity size: 0.1ml （1mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide. Reconstitute with 0.1ml sterile distilled water.）
Background: Met-enkephalin （tyr-gly-gly-phe-met） and leu-enkephalin （tyr-gly-gly-phe-leu） are pentapeptides which compete with and mimic the effects of opiate drugs. Although interest in enkephalins stems largely from their possible role in the brain, the richest source of these peptides is the adrenal gland. The amino acid sequence shows that the precursor is 267 amino acids long and contains 6 interspersed Met-enkephalin sequences and 1 Leu-enkephalin sequence. The precursor does not contain the sequences of dynorphin, alpha-neo-endorphin or beta-endorphin. （Because of structural similarities it had been postulated that beta-endorphin is precursor of Met-enkephalin, and that dynorphin or alpha-neo-endorphin is precursor of Leu-enkephalin.）
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK（114-133） and PENK（237-258） increase glutamate release in the striatum. PENK（114-133） decreases GABA concentration in the striatum.
Also known as: Enkephalin A; PENK; P ENK; Preproencephalin A; Proenkephalin A; Proenkephalin; Synenkephalin; Opioid growth factor; OGF; Leu enkephalin; Met enkephalin; PENK protein; ProenkephalinA.
Specificity:
Rabbit Polyclonal IgG, affinity purified by Protein A.
Reacts with: hu, mo, rat, dog, hrs, chk
Immunogen: KLH conjugated synthetic peptide derived from human PENK C-terminus.
Predicted molecular weight: 29kDa

Application: WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.

Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The Lyophilized or Liquid antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications