B-27® 添加剂是无血清添加剂，用于海马神经元和其他中枢神经系统（CNS）神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体，可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用，用于神经细胞培养而不需要再添加饲养层细胞。
B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用，我们提供 其他配方的B-27® 添加剂.
质量控制检测
B-27® 添加剂 进行了18天妊娠的原代大鼠（Sprague Dawley） 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。
仅供研究使用。除非另有说明，不得用于任何动物或人类治疗或诊断。
- 面议
参考价：
起订量：
B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书
- 型号
该企业相似产品
Elisa试剂盒,检测试剂盒,进口原装Elisa试剂盒，抗体，培养基，生物试剂，细胞
上海恪敏生物科技有限公司是一家集经销批发、招商代理的有限责任公司
生化部主营：生化试剂、抗体、蛋白/多肽、分子生物学、细胞生物学、Elisa试剂盒、发光试剂盒、培养基、细胞、蛇毒试剂、标准品、 标准溶液……(主营)试剂、正规品牌Elisa kit、抗体蛋白、分子生物、标准品（溶液）……
abcam、BD、Beckman、GE、invitrogen、Qiagen、Roche、sigma、Thermo、Zymo……等等品牌。
化工部主营：标准滴定溶液，PH缓冲液，指示剂等。
详细信息
-
D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.
- 作者
- Congar P; Bergevin A; Trudeau LE
- 杂志
- J Neurophysiol （2002） 87:1046-1102
- 产品用途
- Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）.
- ID:
- PN64425
-
Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.
- 作者
- Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;
- 杂志
- Neuron （2002） 33:789-804
- 产品用途
- Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator.
- ID:
- PN47418
-
Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.
- 作者
- Kabos Peter; Kabosova Andrea; Neuman Toomas;
- 杂志
- J Biol Chem （2002） 277:8763-8769
- 产品用途
- Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen）
- ID:
- PN63471
-
Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.
- 作者
- Lesuisse Christian; Martin Lee J;
- 杂志
- J Neurobiol （2002） 51:9-23
- 产品用途
- A long-term cell culture system was used to study maturation, aging, and death of cortical neurons.
- ID:
- PN60461
-
An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.
- 作者
- Joseph-Bravo Patricia; Pérez-MartÃnez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;
- 杂志
- Brain Res Brain Res Protoc （2002） 9:93-104
- 产品用途
- Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties
- ID:
- PN62028
描述
详细说明
常用规格
| Cell Type: | Neuronal Cells |
| Product Size: | 10 ml |
| Classification: | Serum-Free |
| Other Additives: | Vitamin A, Antioxidant Cocktail, Insulin |
| Purity or Quality Grade: | Cell Culture Grade |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Dry Ice |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
引文和参考文献 （17）
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按细胞系排列的引文
| 细胞系名称 | 有机体类型 | 组织 |
|---|---|---|
| SH-Sy5Y | Human | Brain |
| Fetal neurons | Rat | Brain |
| Neurons （post-natal/adult） | Rat | Brain |
| Neurons （superior cervical ganglia - SCG） | Rat | Brain |
| Neurons （cortical） | Mouse | Other |
| Neural Stem Cells | Rat | Brain |
- abcam、ABI、Axygen、corning、信号转导抗体、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等进口品牌……
- 以下为本公司常备现货产品
*部分 invitrogen 原装产品
| 品牌 | 货号 | 名称 | 规格 |
| invitrogen | 13778-075 | Lipofectamine RNAiMAX | 0.75ml |
| invitrogen | 13778-150 | Lipofectamine RNAiMAX | 1.5ml |
| invitrogen | 11668-027 | Lipofectamine 2000 Reagent | 0.75ml |
| invitrogen | 11668-019 | Lipofectamine 2000 Reagent | 1.5ml |
| invitrogen | 15596-026 | TRlzol ®Reagent | 100ml |
| invitrogen | 15596-018 | TRlzol ®Reagent | 200ml |
| invitrogen | 10296-010 | Trizol ® LS Reagent | 100ml |
| invitrogen | 10296-028 | Trizol ® LS Reagent | 200ml |
| invitrogen | 17504-044 | B-27（黄颜色） | 10ml |
| invitrogen | 12587-010 | B-27（黄颜色）不含维生素A | 10ml |
| invitrogen | 17502-048 | N-2 （粉红颜色） | 5ml |
| invitrogen | 18064-014 | SuperScript® II Reverse Transcriptase | 10,000U |
| invitrogen | 18080-044 | SuperScript® III Reverse Transcriptase | 10,000U |
第二部分 HyClone胎牛血清
| 品牌 | 货号 | 名称 | 规格 |
| HyClone | SH30370.03 | Australian Standard Fetal Bovine Serum | 500ml |
| HyClone | SH30406.02 | FOETAL BOVINE SERUM | 500ml |
| HyClone | SH30401.01 | New Zealand Calf Serum | 500ml |
| HyClone | SH30066.02 | FetalClone® II | 100ml |
| HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml |
| HyClone | SH30118.02 | Newborn Bovine Calf Serum, U.S. Origin | 100ml |
| HyClone | SH30070.02 | FETAL BOVINE SERUM | 100ml |
| HyClone | SH30087.02 | COSMIC CALF® SERUM | 100ml |
| HyClone | SH30070.03E | FETALBOVINESERUM CHARACTERIZSED | 500ml |
| 100ml | |||
| HyClone | SH30070.03 | FETAL BOVINE SERUM | 500ml |
| HyClone | SH30080.03 | FetalClone® I | 500ml |
| HyClone | SH30084.03 | FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN | 500ml |
| HyClone | SH30396.03 | FETAL BOVINE SERUM CHARACTERIZED | 500ml |
| HyClone | SV30087.02 | 南美源胎牛血清 | 500ml |
| HyClone | sv30087.01 | 南美源胎牛血清 | 100ml |
| HyClone | SH30071.03 | FETAL BOVINE SERUM | 500ml |
| HyClone | SH30071.02 | FETAL BOVINE SERUM | 100ml |
| HyClone | SH30080.02 | FetalClone® I | 100ml |
| HyClone | SH30070.02E | FETAL BOVINE SERUM CHARACTERIZSED | 100ml |
| HyClone | SH30079.02 | FETAL BOVINE SERUM | 100ml |
| HyClone | SH30068.02 | CHARCOAL/DEXTRAN TREATED FBS， 活性炭灭活血清 | 100ml |
| HyClone | SH30068.03 | CHARCOAL/DEXTRAN TREATED FBS， 活性炭灭活血清 | 500ml |
| HyClone | SH30066.03 | FetalClone® II | 500ml |
| HyClone | SH30109.03 | HyClone FetalClone® III | 500ml |
| HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml |
| HyClone | SH30088.02 | STANDARD FETAL BOVINE SERUM | 100ml |
| HyClone | SH30079.03 | FETAL BOVINE SERUM | 500ml |
第二部分 Gibco胎牛血清
| 品牌 | 货号 | 名称 | 规格 |
| Gibco | 12662-029 | MSC QUALIFIED FBS 500ML | 500ML |
| Gibco | 10438-026 | FBS, HI 500ML | 500ML |
| Gibco | 10439-024 | FBS, ES CELL QUAL. USDA APPD. 500ML | 500ML |
| Gibco | 10099-141 | FBS, AUSTRALIAN ORIGIN 500ML | 500ML |
| Gibco | 26400-044 | FBS, DIALYZED, USA ORIGIN 500ML | 500ML |
| Gibco | 26140-079 | FBS QUALIFIED, USA ORIGIN 500ML | 500ML |
| Gibco | 16141-079 | FBS, ES CELL QUAL, USA ORIGIN 500ML | 500ML |
| Gibco | 16140-071 | FBS, HI 500ML | 500ML |
| Gibco | 16000-044 | FBS, CERT, USA ORIGIN 500ML | 500ML |
| Gibco | C2027050 | FBS, URUGUAYAN ORIGIN | 500ML |
| Gibco | 12657-029 | 巴西胎牛 | 500ml |
