N-2添加剂是化学成分确定的，不含血清的，基于Bottenstein’s 的N1配方的添加剂。N－2添加剂主要用于Neurobasal®培养基。
它主要被推荐用于成神经细胞瘤以及周围神经系统（PNS）与中枢神经系统（CNS）的原代细胞培养中有丝分裂后期神经元的生长和表达。N－2添加剂可以替代Bottenstein’s的N1添加剂。我们提供的N－2添加剂是100倍的浓缩体。它可用于添加了生长因子bFGF及EGF的Neurobasal®培养基，或DMEM培养基。
了解更多N2添加剂配方。.
- 面议
参考价：
起订量：
N2细胞培养添加剂invitrogen/Gibco（货号17502-048）说明书
- 型号
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Elisa试剂盒,检测试剂盒,进口原装Elisa试剂盒，抗体，培养基，生物试剂，细胞
上海恪敏生物科技有限公司是一家集经销批发、招商代理的有限责任公司
生化部主营：生化试剂、抗体、蛋白/多肽、分子生物学、细胞生物学、Elisa试剂盒、发光试剂盒、培养基、细胞、蛇毒试剂、标准品、 标准溶液……(主营)试剂、正规品牌Elisa kit、抗体蛋白、分子生物、标准品（溶液）……
abcam、BD、Beckman、GE、invitrogen、Qiagen、Roche、sigma、Thermo、Zymo……等等品牌。
化工部主营：标准滴定溶液，PH缓冲液，指示剂等。
详细信息
-
Estrogen lowers Alzheimer beta-amyloid generation by stimulating trans-Golgi network vesicle biogenesis.
- 作者
- Greenfield Jeffrey P; Leung Lawrence W; Cai Dongming; Kaasik Krista; Gross Rachel S; Rodriguez-Boulan Enrique; Greengard Paul; Xu Huaxi;
- 杂志
- J Biol Chem （2002） 277:12128-12164
- 产品用途
- Neuroblastoma （N2a） cells doubly transfected with human APP695 and human wild-type PS1 were maintained in medium containing 200 µg/ml G418 （or 350 µg/ml Zeocin for Rab11 expressing cell lines）. Cells were plated in equal amounts in serum-free Neurobasal media with N2 supplement, 25 mM glutamate, and 0.5 mM L-glutamine on poly-D-lysine-treated 100-mm dishes .
- ID:
- PN63995
-
Oligomeric and fibrillar species of amyloid-beta peptides differentially affect neuronal viability.
- 作者
- Dahlgren Karie N; Manelli Arlene M; Stine W Blaine Jr; Baker Lorinda K; Krafft Grant A; LaDu Mary Jo;
- 杂志
- J Biol Chem （2002） 277:32046-32099
- 产品用途
- Samples were diluted in NuPage sample buffer and separated by SDS-PAGE on a 12% NuPage Bis-Tris gel （Invitrogen）. The protein was transferred to polyvinylidene difluoride membranes （Invitrogen） and then blocked for 1 h in a solution of 5% nonfat dry milk in TBS/Tween 20. Neuro-2A cells - After overnight incubation, the cells were rinsed with serum-free media containing N2 supplements （Invitrogen）
- ID:
- PN62678
描述
详细说明
常用规格
| Cell Type: | Neuronal Tumor Cells, Neuronal Cells |
| Product Size: | 5 ml |
| Classification: | Serum-Free |
| Purity or Quality Grade: | Cell Culture Grade |
| Shipping Condition: | Dry Ice |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
引文和参考文献 （2）
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按细胞系排列的引文
| 细胞系名称 | 有机体类型 | 组织 |
|---|---|---|
| SH-Sy5Y | Human | Brain |
| HT22 | Mouse | Brain |
| Neural Stem Cells | Rat | Brain |
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- abcam、ABI、Axygen、corning、信号转导抗体、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等进口品牌……
- 以下为本公司常备现货产品
*部分 invitrogen 原装产品
| 品牌 | 货号 | 名称 | 规格 |
| invitrogen | 13778-075 | Lipofectamine RNAiMAX | 0.75ml |
| invitrogen | 13778-150 | Lipofectamine RNAiMAX | 1.5ml |
| invitrogen | 11668-027 | Lipofectamine 2000 Reagent | 0.75ml |
| invitrogen | 11668-019 | Lipofectamine 2000 Reagent | 1.5ml |
| invitrogen | 15596-026 | TRlzol ®Reagent | 100ml |
| invitrogen | 15596-018 | TRlzol ®Reagent | 200ml |
| invitrogen | 10296-010 | Trizol ® LS Reagent | 100ml |
| invitrogen | 10296-028 | Trizol ® LS Reagent | 200ml |
| invitrogen | 17504-044 | B-27（黄颜色） | 10ml |
| invitrogen | 12587-010 | B-27（黄颜色）不含维生素A | 10ml |
| invitrogen | 17502-048 | N-2 （粉红颜色） | 5ml |
| invitrogen | 18064-014 | SuperScript® II Reverse Transcriptase | 10,000U |
| invitrogen | 18080-044 | SuperScript® III Reverse Transcriptase | 10,000U |
第二部分 HyClone胎牛血清
| 品牌 | 货号 | 名称 | 规格 |
| HyClone | SH30370.03 | Australian Standard Fetal Bovine Serum | 500ml |
| HyClone | SH30406.02 | FOETAL BOVINE SERUM | 500ml |
| HyClone | SH30401.01 | New Zealand Calf Serum | 500ml |
| HyClone | SH30066.02 | FetalClone® II | 100ml |
| HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml |
| HyClone | SH30118.02 | Newborn Bovine Calf Serum, U.S. Origin | 100ml |
| HyClone | SH30070.02 | FETAL BOVINE SERUM | 100ml |
| HyClone | SH30087.02 | COSMIC CALF® SERUM | 100ml |
| HyClone | SH30070.03E | FETALBOVINESERUM CHARACTERIZSED | 500ml |
| 100ml | |||
| HyClone | SH30070.03 | FETAL BOVINE SERUM | 500ml |
| HyClone | SH30080.03 | FetalClone® I | 500ml |
| HyClone | SH30084.03 | FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN | 500ml |
| HyClone | SH30396.03 | FETAL BOVINE SERUM CHARACTERIZED | 500ml |
| HyClone | SV30087.02 | 南美源胎牛血清 | 500ml |
| HyClone | sv30087.01 | 南美源胎牛血清 | 100ml |
| HyClone | SH30071.03 | FETAL BOVINE SERUM | 500ml |
| HyClone | SH30071.02 | FETAL BOVINE SERUM | 100ml |
| HyClone | SH30080.02 | FetalClone® I | 100ml |
| HyClone | SH30070.02E | FETAL BOVINE SERUM CHARACTERIZSED | 100ml |
| HyClone | SH30079.02 | FETAL BOVINE SERUM | 100ml |
| HyClone | SH30068.02 | CHARCOAL/DEXTRAN TREATED FBS， 活性炭灭活血清 | 100ml |
| HyClone | SH30068.03 | CHARCOAL/DEXTRAN TREATED FBS， 活性炭灭活血清 | 500ml |
| HyClone | SH30066.03 | FetalClone® II | 500ml |
| HyClone | SH30109.03 | HyClone FetalClone® III | 500ml |
| HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml |
| HyClone | SH30088.02 | STANDARD FETAL BOVINE SERUM | 100ml |
| HyClone | SH30079.03 | FETAL BOVINE SERUM | 500ml |
第二部分 Gibco胎牛血清
| 品牌 | 货号 | 名称 | 规格 |
| Gibco | 12662-029 | MSC QUALIFIED FBS 500ML | 500ML |
| Gibco | 10438-026 | FBS, HI 500ML | 500ML |
| Gibco | 10439-024 | FBS, ES CELL QUAL. USDA APPD. 500ML | 500ML |
| Gibco | 10099-141 | FBS, AUSTRALIAN ORIGIN 500ML | 500ML |
| Gibco | 26400-044 | FBS, DIALYZED, USA ORIGIN 500ML | 500ML |
| Gibco | 26140-079 | FBS QUALIFIED, USA ORIGIN 500ML | 500ML |
| Gibco | 16141-079 | FBS, ES CELL QUAL, USA ORIGIN 500ML | 500ML |
| Gibco | 16140-071 | FBS, HI 500ML | 500ML |
| Gibco | 16000-044 | FBS, CERT, USA ORIGIN 500ML | 500ML |
| Gibco | C2027050 | FBS, URUGUAYAN ORIGIN | 500ML |
| Gibco | 12657-029 | 巴西胎牛 | 500ml |
