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起订量：
人肿瘤坏死因子配体相关分子-1A/Human VEGI（TL1A）
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详细信息
VEGI Human
Human Vascular Endothelial Growth Inhibitor Recombinant
人肿瘤坏死因子配体相关分子-1A
Catalog No. CYT-517
Synonyms:
Tumor necrosis factor ligand superfamily member 15, TNFSF-15, TNFSF15, TNF ligand-related molecule 1, VEGI, TL-1, TL1, TL1A, VEGI192A, VEGI-192, MGC129934, MGC129935.
Introduction:
TNFSF15 is a cytokine that belongs to the tumor necrosis factor （TNF） ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of TNFSF15 is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. TNFSF15 is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. An additional isoform encoded by an alternatively spliced transcript variant has been reported but the sequence of this transcript has not been determined.
Description:
TNFSF15 Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 21.8 kDa.
The TNFSF15 is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation:
The TNFSF15 was lyophilized from a concentrated （1 mg/ml） solution containing 0.5M NaCl and 50mM Tris-HCl pH-7.5.
Solubility:
It is recommended to reconstitute the lyophilized TNFSF15 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
TNFSF15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGI should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Leu-Thr-Lys.
Biological Activity:
The ED50 as determined by the dose-dependant inhibition of the proliferation of HUVEC （Human Umbilical Vein Endothelial Cells） is less then 5μg/ml.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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