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abs04123 Recombinant Human Interferon γ/IFN-γ (E. coli)
- 型号
- abs04123
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爱必信（上海）生物科技有限公司是一家从事生物学试剂及耗材的高新技术企业。公司秉承“为生命科学创造便利！为客户创造价值！为员工创造幸福人生！”的发展理念，拥有专业的产品研发质检团队、市场营销团队及*仓储物流等管理团队。荣获2021年度上海市“专精特新”企业称号。
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详细信息
产品描述：Recombinant Human Interferon gamma is produced by our E.coli expression system and the target gene encoding Gln24-Gln166 is expressed.
别名：Interferon γ蛋白, IFN-γ蛋白;Interferon Gamma, IFN-Gamma, Immune Interferon, IFNG
来源：Escherichia coli.
形态：Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5%Mannitol, 0.1%Tween80, 5%Trehalose, pH6.0.
序列：MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQ SIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKR KRSQMLFRGRRASQ
纯度：Greater than 95% as determined by reducing SDS-PAGE.
内毒素水平：Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
活性：Measured by a cytotoxicity assay using HT-29 cells.The ED50 for this effect is 17 pg/mL.
溶解方法：Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法：Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景说明：IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.
