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人1,5-脱水葡萄糖醇/1,5-脱水山梨(1,5-AG)ELISA Kit

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上海希美化学有限公司

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ELISA KIT,酶免试剂盒,酶联免疫试剂盒,重组蛋白,抗体

上海希美化学有限公司有限公司是一家专业代理销售生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器的高科技企业。产品涉及分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域,同时我们提供专业的生物技术服务。
     
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公司在重视产品质量的同时,也建立了一套集技术支持、物流、售后服务等多个部门的*的服务体系,努力把我们方便、快捷、周到的服务提供给每一个客户。主要代理产品:试剂盒:ELISA试剂盒、免疫组化试剂盒、分子生物学试剂盒、细胞凋亡试剂盒。

ELISA KIT :CUSABIO品牌涵盖37个种属多达五千多种的Elisa试剂盒;拥有广泛的细胞因子、生长因子、趋化因子、激素、酶、病毒抗原和其他许多重组蛋白的蛋白表达系统。

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详细信息

产品名称: 人1,5-脱水葡萄糖醇/1,5-脱水山梨(1,5-AG)ELISA Kit
 
 英文名称: Human 1,5-anhydroglucitol (1,5-AG) ELISA kit
 
 货号: CSB-E08988h
 
 规格: 96T
 
 种属: Human
 
 待测物名称: 1,5-anhydroglucitol,1,5-AG
 
 缩写: 1,5AG
 
 样本类型: serum, plasma, tissue homogenates
 
 检测范围: 28.5 nmol/ml-500 nmol/ml
 
 灵敏度: 17.8 nmol/ml
 
 反应时间: 1-5h
 
 所需样本体积: 50-100ul
 
 检测波长: 450 nm
 
 用途: For research use only. Not for diagnostic use.
 
 精密度: Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 
 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
 
 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
 
 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IGF1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
  
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