研域生物 品牌
生产厂家厂商性质
上海市所在地
本产品仅用于科研,不用于临床
人白介素1可溶性受体Ⅰ酶免试剂盒,(IL-1sRⅠ)ELISA检测试剂盒,英文名:IL-1sRⅠ ELISA Kit,英文缩写:IL-1sRⅠ ,常见试剂盒规格:96T/48T,产品别名:人白介素1可溶性受体ⅠELISA检测试剂盒,IL-1sRⅠ 检测试剂盒,IL-1sRⅠ 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人白介素1可溶性受体Ⅰ酶免试剂盒,(IL-1sRⅠ)ELISA检测试剂盒 操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
兔热休克蛋白20(HSP-20)ELISA试剂盒Rabbit Heat Shock Protein 20,HSP-20 ELISA KitY0315196T/48T
兔β淀粉样蛋白1-40(Aβ1-40)ELISA试剂盒Rabbit amyloid beta peptide 1-40,Aβ1-40 ELISA KitY0315296T/48T
兔组织因子途径抑制物(TFPI)ELISA试剂盒Rabbit Tissue factor pathway inhibitor,TFPI ELISA KitY0315396T/48T
兔白介素17(IL-17)ELISA试剂盒Rabbit Interleukin 17,IL-17 ELISA KitY0315496T/48T
兔乙酰胆碱(ACh)ELISA试剂盒Rabbit acetylcholine,ACH ELISA KitY0315596T/48T
兔去甲肾上腺素(NA)ELISA试剂盒Rabbit Noradrenaline,NA ELISA KitY0315696T/48T
兔子生长激素释放多肽(GHRP)ELISA试剂盒Rabbit Growth Hormone Releasing Peptide,GHRP ELISA Kit Y0315796T/48T
兔子α干扰素(IFN-α)ELISA试剂盒 Rabbit Interferon α,IFN-α ELISA Kit Y0315896T/48T
兔子E选择素(E-Selectin/CD62E)ELISA试剂盒 Rabbit E-Selectin ELISA Kit Y0315996T/48T
兔子视黄醇结合蛋白4(RBP-4)ELISA试剂盒Rabbit Retinol binding protein 4,RBP-4 ELISA Kit Y0316096T/48T
兔子主要组织相容性复合体Ⅲ类(MHCⅢ/RLAⅢ)ELISA试剂盒Rabbit major histocompatibility complex Ⅲ,MHCⅢ/RLAⅢ ELISA Kit Y0316196T/48T
兔淋巴细胞功能相关抗原3(LFA-3/CD58)ELISA试剂盒Rabbit lymphocyte function associated antigen 3,LFA-3 ELISA Kit Y0316296T/48T
兔儿茶酚胺(CA)ELISA试剂盒Rabbit catecholamine,CA ELISA Kit Y0316396T/48T
兔吡啶交联物(PY)ELISA试剂盒Rabbit pyridinium crosslink/PyriLinks,PY ELISA Kit Y0316496T/48T
兔糖化血红蛋白A1c(GHbA1c)ELISA试剂盒Rabbit Glycated hemoglobin A1c,GHbA1c ELISA Kit Y0316596T/48T
兔半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)ELISA试剂盒Rabbit Cystatin C,Cys-C ELISA Kit Y0316696T/48T
兔子脱氧吡啶酚/脱氧吡啶啉(DPD)ELISA试剂盒deoxypyridinoline,DPD ELISA Kit Y0316796T/48T
兔子组织因子(TF)ELISA试剂盒rabbit Tissue factor,TF ELISA KitY0316896T/48T
兔子骨胶原交联(Cr)ELISA试剂盒Rabbit Crosslaps,Cr ELISA Kit Y0316996T/48T
兔子组织型纤溶酶原激活剂(t-PA)ELISA试剂盒 rabbit Tissue-type Plasiminogen Actilyse,t-PA ELISA KitY0317096T/48T
兔子组织多肽抗原(TPA)ELISA试剂盒rabbit Tissue Polypeptide Antigen,TPA ELISA Kit Y0317196T/48T
兔子组胺(HIS)ELISA试剂盒rabbit Histamine,HIS ELISA KitY0317296T/48T
兔子转化生长因子β1(TGF-β1)ELISA试剂盒rabbit Transforming Growth factor β1,TGF-β1 ELISA KitY0317396T/48T
兔子肿瘤坏死因子α(TNF-α) ELISA试剂盒 rabbit Tumor necrosis factor α,TNFα ELISA Kit Y0317496T/48T
兔子脂联素(ADP)ELISA试剂盒rabbit adiponectin,ADP ELISA KitY0317596T/48T
兔肾素(Renin)ELISA试剂盒Rabbit Renin ELISA Kit Y0317696T/48T
兔子胰岛素样生长因子2(IGF-2)ELISA试剂盒rabbit Insulin-like growth factor 2,IGF-2 ELISA Kit Y0317796T/48T
兔子胰岛素样生长因子1(IGF-1)ELISA试剂盒rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit Y0317896T/48T
兔子胰岛素(INS)ELISA试剂盒rabbit Insulin,INS ELISA Kit Y0317996T/48T
兔子氧化低密度脂蛋白(OxLDL)ELISA试剂盒Rabbit oxidized lowdensity lipoprotein,OxLDL ELISA Kit Y0318096T/48T
兔子补体蛋白4(C4)ELISA试剂盒Rabbit Complement 4,C4 ELISA Kit Y0318196T/48T
兔子血小板衍生生长因子(PDGF)ELISA试剂盒rabbit plaet-derived growth factor,PDGF ELISA Kit Y0318296T/48T
兔过氧化物酶体增殖因子活化受体γ(PPAR-γ)ELISA试剂盒Rabbit Peroxisome Proliferator-activated receptor γ,PPAR-γ ELISA Kit Y0318396T/48T
兔子血小板活化因子(PAF)ELISA试剂盒rabbit plaet activating factor,PAF ELISA Kit Y0318496T/48T
兔子血栓调节蛋白(TM)ELISA试剂盒 rabbit thrombomodulin,TM ELISA Kit Y0318596T/48T
兔子血纤蛋白原降解产物(FDP)ELISA试剂盒rabbit Fibrinogen Degradation Product,FDP ELISA Kit Y0318696T/48T
兔子纤溶酶原激活物抑制因子(PAI)ELISA试剂盒rabbit plasminogen activator inhibitor,PAI ELISA Kit Y0318796T/48T
兔子纤溶酶原激活物抑制因子1(PAI-1)ELISA试剂盒rabbit plasminogen activator inhibitor 1,PAI-1 ELISA Kit Y0318896T/48T
兔子细胞间粘附分子3(ICAM-3/CD50)ELISA试剂盒rabbit intercellular adhesion molecule 3,ICAM-3 ELISA Kit Y0318996T/48T
兔子葡萄糖依赖性胰岛素释放多肽(GIP)ELISA试剂盒Rabbit Glucose dependent insulin releasing polypeptide,GIP ELISA Kit Y0319096T/48T
兔子细胞间粘附分子2(ICAM-2/CD102)ELISA试剂盒rabbit intercellular adhesion molecule 2,ICAM-2 ELISA KitY0319196T/48T
兔子细胞间粘附分子1(ICAM-1/CD54)ELISA试剂盒rabbit intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit Y0319296T/48T
兔子透明质酸(HA)ELISA试剂盒rabbit Hyaluronic acid,HA ELISA Kit Y0319396T/48T
兔子瘦素(LEP)ELISA试剂盒rabbit Leptin,LEP ELISA Kit Y0319496T/48T
兔子神经营养因子4(NT-4)ELISA试剂盒rabbit neurotrophin 4,NT-4 ELISA Kit Y0319596T/48T
兔子神经营养因子3(NT-3)ELISA试剂盒rabbit neurotrophin 3,NT-3 ELISA Kit Y0319696T/48T
兔子神经特异性烯醇化酶(NSE)ELISA试剂盒rabbit Neuron-specific enolase,NSE ELISA KitY0319796T/48T
兔子神经生长因子(NGF)ELISA试剂盒 rabbit Nerve growth factor,NGF ELISA KitY0319896T/48T
兔子髓磷脂碱性蛋白(MBP)ELISA试剂盒Rabbit myelin basic protein,MBP ELISA Kit Y0319996T/48T
兔子前列腺素E2(PGE2)ELISA试剂盒rabbit Prostaglandin E2,PG-E2 ELISA KitY0320096T/48T
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。