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人白介素16酶免试剂盒,(IL-16)ELISA检测试剂盒,英文名:IL-16 ELISA Kit,英文缩写:IL-16 ,常见试剂盒规格:96T/48T,产品别名:人白介素16ELISA检测试剂盒,IL-16 检测试剂盒,IL-16 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。人白介素16酶免试剂盒,(IL-16)ELISA检测试剂盒 操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
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兔子皮质酮/肾上腺酮(CORT)ELISA试剂盒rabbit Corticosterone,CORT ELISA KitY0320296T/48T
兔子皮质醇(Cortisol)ELISA试剂盒Rabbit Cortisol ELISA Kit Y0320396T/48T
兔子脑钠素/脑钠尿肽(BNP)ELISA试剂盒rabbit brain natriuretic peptide,BNP ELISA KitY0320496T/48T
兔子内皮抑素(ES)ELISA试剂盒rabbit Endostatin,ES ELISA Kit Y0320596T/48T
兔子内皮素1(ET-1)ELISA试剂盒rabbit Endothelin 1,ET-1 ELISA Kit Y0320696T/48T
兔子免疫球蛋白G(IgG)ELISA试剂盒 rabbit Immunoglobulin G,IgG ELISA KitY0320796T/48T
兔子免疫球蛋白E(IgE)ELISA试剂盒rabbit Immunoglobulin E,IgE ELISA KitY0320896T/48T
兔子免疫球蛋白A(IgA)ELISA试剂盒 rabbit Immunoglobulin A,IgA ELISA Kit Y0320996T/48T
兔子可溶性血管细胞粘附分子1(sVCAM-1)ELISA试剂盒Rabbit soluble Vascuolar cell adhesion molecule 1,sVCAM-1 ELISA KitY0321096T/48T
兔子可溶性细胞间粘附分子1(sICAM-1)ELISA试剂盒rabbit Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit Y0321196T/48T
兔子可溶性E选择素(sE-selectin)ELISA试剂盒rabbit soluble E-selectin,sE-selectin ELISA KitY0321296T/48T
兔子巨噬细胞炎性蛋白5(MIP-5)ELISA试剂盒rabbit macrophage inflammatory protein 5,MIP-5 ELISA Kit Y0321396T/48T
兔子碱性成纤维细胞生长因子(bFGF)ELISA试剂盒 rabbit basic fibroblast growth factor,bFGF ELISA Kit Y0321496T/48T
兔子免疫球蛋白M(IgM)ELISA试剂盒 rabbit Immunoglobulin M,IgM ELISA Kit Y0321596T/48T
兔子甲状腺素(T4)ELISA试剂盒rabbit thyroxine,T4 ELISA Kit Y0321696T/48T
兔子基质金属蛋白酶抑制因子1(TIMP-1)ELISA试剂盒rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA KitY0321796T/48T
兔子基质金属蛋白酶9(MMP-9)ELISA试剂盒rabbit matrix metalloproteinase 9,MMP-9 ELISA Kit Y0321896T/48T
兔子基质金属蛋白酶4(MMP-4)ELISA试剂盒rabbit matrix metalloproteinase 4,MMP-4 ELISA KitY0321996T/48T
兔子基质金属蛋白酶5(MMP-5)ELISA试剂盒rabbit matrix metalloproteinase 5,MMP-5 ELISA KitY0322096T/48T
兔子睾酮(T)ELISA试剂盒rabbit Testoterone,T ELISA Kit Y0322196T/48T
兔子肝细胞生长因子(HGF)ELISA试剂盒rabbit hepatocyte growth factor,HGF ELISA Kit Y0322296T/48T
兔子γ干扰素(IFN-γ)ELISA试剂盒 rabbit Interferon γ ,IFN-γ ELISA Kit Y0322396T/48T
兔子端粒酶(TE)ELISA试剂盒rabbit omerase,TE ELISA Kit Y0322496T/48T
兔子单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA试剂盒protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA KitY0322596T/48T
兔子促肾上腺皮质激素(ACTH)ELISA试剂盒rabbit adrencocorticotropic hormone,ACTH ELISA KitY0322696T/48T
兔子促卵泡素(FSH)ELISA试剂盒rabbit follicle-stimulating hormone,FSH ELISA Kit Y0322796T/48T
兔子促甲状腺素(TSH)ELISA试剂盒rabbit Thyroid Stimulating Hormone,TSH ELISA Kit Y0322896T/48T
兔子雌二醇(E2)ELISA试剂盒rabbit Estradiol,E2 ELISA KitY0322996T/48T
兔子肠脂肪酸结合蛋白(iFABP)ELISA试剂盒rabbit intestinal fatty acid binding protein,iFABP ELISA Kit Y0323096T/48T
兔子表皮生长因子(EGF)ELISA试剂盒 Rabbit Epidermal growth factor,EGF ELISA Kit Y0323196T/48T
兔子白血病抑制因子受体(LIFR)ELISA试剂盒rabbit leukemia inhibitory factor receptor,LIFR ELISA Kit Y0323296T/48T
兔子白三烯B4(LTB4)ELISA试剂盒rabbit Leukotriene B4,LT-B4 ELISA Kit Y0323396T/48T
兔子白介素8(IL-8/CXCL8)ELISA试剂盒rabbit Interleukin 8,IL-8 ELISA KitY0323496T/48T
兔子白介素6(IL-6)ELISA试剂盒rabbit Interleukin 6,IL-6 ELISA KitY0323596T/48T
兔子白介素4(IL-4)ELISA试剂盒rabbit Interleukin 4,IL-4 ELISA Kit Y0323696T/48T
兔子白介素3(IL-3)ELISA试剂盒Rabbit Interleukin 3,IL-3 ELISA Kit Y0323796T/48T
兔子白介素1β (IL-1β)ELISA试剂盒Rabbit Interleukin 1β,IL-1β ELISA Kit Y0323896T/48T
兔肌钙蛋白Ⅰ(Tn-Ⅰ)ELISA试剂盒Rabbit Troponin Ⅰ,Tn-Ⅰ ELISA Kit Y0323996T/48T
兔子白介素10(IL-10)ELISA试剂盒Rabbit Interleukin 10,IL-10 ELISA KitY0324096T/48T
兔子白介素1(IL-1)ELISA试剂盒Rabbit Interleukin 1,IL-1 ELISA KitY0324196T/48T
兔子S100B蛋白(S-100B)ELISA试剂盒rabbit Soluble protein-100B,S-100B ELISA KitY0324296T/48T
兔子S100蛋白(S-100)ELISA试剂盒rabbit Soluble protein-100,S-100 ELISA KitY0324396T/48T
兔主要组织相容性复合体Ⅱ类(MHCⅡ/RLAⅡ)ELISA试剂盒Rabbit major histocompatibility complexⅡ,MHCⅡ/RLAⅡ ELISA Kit Y0324496T/48T
兔子凝血酶受体(TR)ELISA试剂盒Rabbit thrombin receptor,TR ELISA Kit Y0324596T/48T
兔子可溶性P选择素(sP-selectin)ELISA试剂盒rabbit soluble P-selectin,sP-selectin ELISA KitY0324696T/48T
兔子Ⅰ型前胶原羧基端肽(PⅠCP)ELISA试剂盒rabbit Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA KitY0324796T/48T
兔子Ⅰ型胶原(Col Ⅰ)ELISA试剂盒rabbit Collagen Type Ⅰ,Col Ⅰ ELISA KitY0324896T/48T
兔子Ⅱ型胶原(Col Ⅱ)ELISA试剂盒rabbit Collagen Type Ⅱ,Col Ⅱ ELISA KitY0324996T/48T
兔子Ⅲ型前胶原肽(PⅢNP)ELISA试剂盒rabbit N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA KitY0325096T/48T
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。