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小鼠肺部活化调节趋化因子酶免试剂盒,(PARC/CCL18)ELISA检测试剂盒 ,英文名:PARC/CCL18 ELISA Kit ,英文缩写:PARC/CCL18 ,常见试剂盒规格:96T/48T,产品别名:小鼠肺部活化调节趋化因子ELISA检测试剂盒,PARC/CCL18 检测试剂盒, PARC/CCL18 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠肺部活化调节趋化因子酶免试剂盒,(PARC/CCL18)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不*,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
Misato同源物1(MSTO1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Misato Homolog 1 (MSTO1)48T/96TY13985Mus musculus (Mouse)
蛋白酶3(PR3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Proteinase 3 (PR3)48T/96TY13986Mus musculus (Mouse)
轻肽神经丝蛋白(NEFL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Neurofilament, Light Polypeptide (NEFL)48T/96TY13987Mus musculus (Mouse)
DNA激活蛋白激酶催化亚基肽(PRKDC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC)48T/96TY13988Mus musculus (Mouse)
酰基羧酸水解酶(AOAH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Acyloxyacyl Hydrolase (AOAH)48T/96TY13989Homo sapiens (Human)
龙虾肽酶样金属内肽酶(ASTL)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Astacin Like Metallo Endopeptidase (ASTL)48T/96TY13990Homo sapiens (Human)
酪蛋白β(CSN2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Casein Beta (CSN2)48T/96TY13991Bos taurus; Bovine (Cattle)
半胱氨酸丰富分泌蛋白3(CRISP3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cysteine Rich Secretory Protein 3 (CRISP3)48T/96TY13992Homo sapiens (Human)
2',5'-寡腺苷酸合成酶2(OAS2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 2 (OAS2)48T/96TY13993Homo sapiens (Human)
2',5'-寡腺苷酸合成酶3(OAS3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 2',5'-Oligoadenylate Synthetase 3 (OAS3)48T/96TY13994Homo sapiens (Human)
降钙素原(PCT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Procalcitonin (PCT)48T/96TY13995Rattus norvegicus (Rat)
白介素10受体α(IL10Rα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 10 Receptor Alpha (IL10Ra)48T/96TY13996Homo sapiens (Human)
死亡关联蛋白激酶1(DAPK1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Death Associated Protein Kinase 1 (DAPK1)48T/96TY13997Mus musculus (Mouse)
成纤维细胞激活蛋白α(FAPα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibroblast Activation Protein Alpha (FAPa)48T/96TY13998Homo sapiens (Human)
二酰甘油-O-酰基转移酶同源物1(DGAT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1)48T/96TY13999Rattus norvegicus (Rat)
大肠杆菌宿主残留蛋白(ECP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Escherichia coli Protein (ECP)48T/96TY14000General
纤维蛋白原相关蛋白1(FGL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibrinogen Like Protein 1 (FGL1)48T/96TY14001Mus musculus (Mouse)
剪切修复交叉互补修复缺陷偶联因子1(ERCC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1)48T/96TY14002Homo sapiens (Human)
死亡关联蛋白激酶3(DAPK3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Death Associated Protein Kinase 3 (DAPK3)48T/96TY14003Mus musculus (Mouse)
核有丝分裂器蛋白1(NUMA1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nuclear Mitotic Apparatus Protein 1 (NUMA1)48T/96TY14004Homo sapiens (Human)
S100钙结合蛋白A2(S100A2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for S100 Calcium Binding Protein A2 (S100A2)48T/96TY14005Homo sapiens (Human)
血管紧张素1-7(Ang1-7)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Angiotensin 1-7 (Ang1-7)48T/96TY14006Mus musculus (Mouse)
驱动蛋白家族成员14(KIF14)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Kinesin Family, Member 14 (KIF14)48T/96TY14007Homo sapiens (Human)
V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)48T/96TY14008Mus musculus (Mouse)
分泌粒蛋白Ⅲ(SCG3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secretogranin III (SCG3)48T/96TY14009Homo sapiens (Human)
白细胞关联免疫球蛋白样受体1(LAIR1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1)48T/96TY14010Homo sapiens (Human)
鸟氨酸脱羧酶(ODC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ornithine Decarboxylase (ODC)48T/96TY14011Mus musculus (Mouse)
表皮转谷氨酰胺酶(TGM3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transglutaminase 3, Epidermal (TGM3)48T/96TY14012Mus musculus (Mouse)
角质转谷氨酰胺酶(TGM1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Transglutaminase 1, Keratinocyte (TGM1)48T/96TY14013Mus musculus (Mouse)
干扰素α(IFNα)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY14014Ovis aries; Ovine (Sheep)
凋亡蛋白酶激活因子1(APAF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)48T/96TY14015Mus musculus (Mouse)
凋亡蛋白酶激活因子1(APAF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)48T/96TY14016Rattus norvegicus (Rat)
白介素31(IL31)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 31 (IL31)48T/96TY14017Homo sapiens (Human)
多效生长因子(PTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pleiotrophin (PTN)48T/96TY14018Bos taurus; Bovine (Cattle)
多效生长因子(PTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pleiotrophin (PTN)48T/96TY14019Mus musculus (Mouse)
多效生长因子(PTN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Pleiotrophin (PTN)48T/96TY14020Rattus norvegicus (Rat)
丝切蛋白1(CFL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cofilin 1, Non Muscle (CFL1)48T/96TY14021Mus musculus (Mouse)
丝切蛋白1(CFL1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cofilin 1, Non Muscle (CFL1)48T/96TY14022Rattus norvegicus (Rat)
淋巴毒素β(LTβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lymphotoxin Beta (LTb)48T/96TY14023Mus musculus (Mouse)
淋巴毒素β(LTβ)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lymphotoxin Beta (LTb)48T/96TY14024Rattus norvegicus (Rat)
溶酶体关联膜蛋白1(LAMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lysosomal Associated Membrane Protein 1 (LAMP1)48T/96TY14025Homo sapiens (Human)
CD200受体2(CD200R2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for CD200 Receptor 2 (CD200R2)48T/96TY14026Homo sapiens (Human)
胆绿素还原酶B(BLVRB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Biliverdin Reductase B (BLVRB)48T/96TY14027Homo sapiens (Human)
胆绿素还原酶A(BLVRA)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Biliverdin Reductase A (BLVRA)48T/96TY14028Homo sapiens (Human)
粒细胞趋化蛋白2(GCP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Granulocyte Chemotactic Protein 2 (GCP2)48T/96TY14029Mus musculus (Mouse)
二级淋巴组织趋化因子(SLC)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secondary Lymphoid Tissue Chemokine (SLC)48T/96TY14030Mus musculus (Mouse)
B-淋巴细胞趋化因子1(BLC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Lymphocyte Chemoattractant 1 (BLC1)48T/96TY14031Mus musculus (Mouse)
B-淋巴细胞趋化因子1(BLC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for B-Lymphocyte Chemoattractant 1 (BLC1)48T/96TY14032Rattus norvegicus (Rat)
胸肾表达趋化因子(BRAK)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Breast And Kidney Expressed Chemokine (BRAK)48T/96TY14033Rattus norvegicus (Rat)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。