10% BSA Diluent/Blocking Solution serves a dual purpose.
As a blocking solution it binds to any remaining protein
binding sites after the primary antigen or antibody is bound to
the solid phase surface. As a diluent for antigens, antibodies,
and other proteins, it ensures minimal non-specific interaction
of these reactants with each other and with the solid phase
surface.

FORM/STORAGE/STABILITY
Catalog No. 50-61-00 consists of 2 x 100 mL （Catalog No. 50-61-
01） 10% BSA Diluent/Blocking Solution.
Store at 2 - 8°C. Stable for a minimum of one year from date of
receipt when stored at 2 - 8°C. If crystals appear upon
refrigeration, warm the bottle to 37°C with mixing to dissolve.

CONTENT
Solution contains 10% Bovine
Serum Albumin （BSA） in a phosphate buffer.

USE
Preparation:
Before use, dilute with reagent quality water as follows.
Prepare enough blocking solution to compley cover the
treated surface.

Blocking Diluent
ELISA 1:10
（i.e. 1 mL 10% BSA
Diluent/Blocking
Solution + 9 mL water）
1:15
（i.e. 1 mL 10% BSA
Diluent/Blocking
Solution + 14 mL
water）
Blotting 1:10
（i.e. 1 mL 10% BSA
Diluent/Blocking
Solution + 9 mL water）
1:10
（i.e. 1 mL 10% BSA
Diluent/Blocking
Solution + 9 mL water）