Caspase-9也称ICE-LAP6或Mch6，可与细胞*素c和Apaf1形成复合物被激活，并进一步激活细胞凋亡的最关键酶caspase-3，从而触发凋亡级联反应。因此，Caspase-9是细胞凋亡信号转导过程中重要的上游caspase。
细胞凋亡属于程序化细胞死亡，可见于各种器官和细胞，在正常发育、生理、病理过程中对细胞和器官的稳态具有十分重要的调节作用。Caspase (Cysteine-requiring Aspartate Protease)是参与细胞凋亡过程的蛋白酶家族，包含10多个成员。Caspase-9也称ICE-LAP6或Mch6，可与细胞*素c和Apaf1形成复合物被激活，并进一步激活细胞凋亡的最关键酶caspase-3，从而触发凋亡级联反应。因此，Caspase-9是细胞凋亡信号转导过程中重要的上游caspase。其激活可以通过磷酸化进行调控。试剂盒测定原理基于Caspase-9特异水解其多肽底物Ac-LEHD-pNA (N-acetyl-Leu-Glu-His-Asp-p-nitroanilide)，释放出游离的硝基ben胺pNA，后者呈黄色在405 nm具有最大吸收峰，用可见光分光光度比色方法测定。其吸光度值对应于Caspase-9的水解活性。反应体系100微升，试剂盒提供的试剂，除标准曲线外可进行50或100次样品测定。适用：测定哺乳动物组织、细胞caspase-9活性。

组成与储存：
1. 裂解缓冲液： 20 ml ，4 ºC
2. 反应缓冲液： 10 ml ，4 ºC
3. 2 mM Ac-LEHD-pNA底物： 0.5 ml，－20 ºC避光
4. 10 mM pNA标准品： 0.5 ml，－20 ºC避光
以上标出的量为100次检测，50次检测的试剂量减半。试剂常温运输。到达后按要求储存，1年内稳定。

所需仪器：
酶标仪与96孔酶标板；或可见光分光光度计配100μl容积的石英比色杯。
工作波长：最大吸收波长405 nm，如不具备也可在400~450 nm范围内测定。

相关文献：
《Overexpression of long noncoding RNA LINC01297 indicates an unfavorable prognosis and promotes tumorigenesis in breast cancer》 作者:Min Jiang,Yu Xiao,Deshui Liu,Na Luo,Qi Gao,Yueyao Guan 期刊:Gene 影响因子:2.638 PMID:29981416
《Long non-coding RNA HOTAIR promotes tumorigenesis and forecasts a poor prognosis in cholangiocarcinoma》 作者:WeiQin,Pengcheng Kang, YiXu, Kaiming Leng, Zhenglong Li, Lining Huang,JianjunGao, YunfuCui & XiangyuZhong 期刊:Scientific Reports 影响因子:4.011 PMID:30111807
《Transient receptor potential ankyrin 1 (trpa1) mediates il-2β-induced apoptosis in rat chondrocytes via calcium overload and mitochondrial dysfunction》 作者:Songjiang Yin, Li Zhang, Liang Ding, Zhengquan Huang, Bo Xu, XiaoChen Li, Peimin Wang & Jun Mao  期刊:Journal of Inflammation 影响因子:2.662 PMID:30564065
《SP1-induced upregulation of lncRNA SPRY4-IT1 exerts oncogenic properties by scaffolding EZH2/LSD1/DNMT1 and sponging miR-101-3p in cholangiocarcinoma》 作者:Yi Xu, Yue Yao, Xingming Jiang, Xiangyu Zhong, Zhidong Wang, Chunlong Li, Pengcheng Kang, Kaiming Leng, Daolin Ji, Zhenglong Li, Lining Huang, Wei Qin & Yunfu Cui  期刊:Journal of Experimental & Clinical Cancer Research 影响因子:5.646 PMID:29642935
《Long non-coding RNA CCAT2 promotes cholangiocarcinoma cells migration and invasion by induction of epithelial-to-mesenchymal transition》 作者:Yi Xu,Yue Yao,Wei Qin,Xiangyu Zhong,Xingming Jiang,Yunfu Cui 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:29329034
《Overexpression of hsa_circ_0007534 predicts unfavorable prognosis for osteosarcoma and regulates cell growth and apoptosis by affecting AKT/GSK-4β signaling pathway》 作者:Baoquan Li，Xiaoguang Li 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:30142548
《Decreased circular RNA hsa_circ_0001649 predicts unfavorable prognosis in glioma and exerts oncogenic properties in vitro and in vivo》 作者:Yu Wang,Xin Sui,Hong Zhao,Ling Cong,Yu Li,Ting Xin,Mingxing Guo,Wenbo Hao 期刊:Gene 影响因子:2.638 PMID:30016668
《Long non-coding RNA HOTTIP is upregulated in renal cell carcinoma and regulates cell growth and apoptosis by epigenetically silencing of LATS3》 作者:Feifei Peng,Xiaoli Shi,Yin Meng,Bo Dong,Guangchi Xu,Tingting Hou,Yang Shi,Tao Liu 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:30021349
《Upregulation of circular RNA hsa_circ_0007534 predicts unfavorable prognosis for NSCLC and exerts oncogenic properties in vitro and in vivo》 作者:Yi Qi,Bingquan Zhang,Junting Wang,Mengna Yao 期刊:Gene 影响因子:2.638 PMID:30017736
《Upregulated long non-coding RNA SPRY4-IT1 predicts dismal prognosis for pancreatic ductal adenocarcinoma and regulates cell proliferation and apoptosis》 作者:Yue Yao,Ping Gao,Lili Chen,Wei Wang,Jinchao Zhang,Qiang Li,Yi,Xu 期刊:Gene 影响因子:2.638 PMID:29551494
《SP1-induced upregulation of lncRNA PANDAR predicts adverse phenotypes in retinoblastoma and regulates cell growth and apoptosis in vitro and in vivo》 作者:Lijie Sheng,Jing Wu,Xuewu Gong,Duo Dong,Xiaoxue Sun 期刊:Gene 影响因子:2.638 PMID:29778422
《Enhanced expression of lncRNA TP73-AS1 predicts unfavorable prognosis for gastric cancer and promotes cell migration and invasion by induction of EMT》 作者:Wei Zhang，Yajuan Zhai，Wenbin Wang，Min Cao，Chunjie Ma 期刊:Gene 影响因子:2.638 PMID:30118890
《A novel prognostic biomarker for pancreatic ductal adenocarcinoma: hsa_circ_0001650》 作者:Yuehong Jiang,Tong Wang,Li Yan,Lijuan Qu 期刊:Gene 影响因子:2.638 PMID:29969694
《Enhanced expression of lncRNA TP73-AS1 predicts adverse phenotypes for cholangiocarcinoma and exerts oncogenic properties in vitro and in vivo》 作者:Yue Yao, Yue Sun,Yuehong Jiang,Lijun Qu,Yi Xu 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:29966969
《Upregulated circular RNA circ_0007534 indicates an unfavorable prognosis in pancreatic ductal adenocarcinoma and regulates cell proliferation, apoptosis, and invasion by sponging miR-625 and miR-892b.》 作者:Liguo Hao  Wei Rong  Lianjie Bai  Hongsheng Cui  Shuli Zhang  Yuanchun Li  Datong Chen  Xin Meng 期刊:J. Cell. Biochem. 影响因子:3.448 PMID:30382592
《Upregulated long noncoding RNA LINC01296 indicates a dismal prognosis for pancreatic ductal adenocarcinoma and promotes cell metastatic properties by affecting EMT.》 作者:Qihua Yuan  Yuxia Zhang  Lijun Feng  Yuehong Jiang 期刊:J. Cell. Biochem. 影响因子:3.448 PMID:30203487
 

Caspase-9活性检测试剂盒 Caspase-9活性检测试剂盒