Preparation :

Produced in Sprague Dawley rats using highly pure （>98%） recombinant murine IL-12 as the immunizing antigen. This IgG1_K antibody was purified from ascites fluid by Protein A affinity chromatography.

Catalog #:

500-M59

Source :

Rats

Formulation :

A sterile filtered antibody solution was lyophilized from PBS.

Stability :

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20^o C. Frozen aliquots are stable for at least six weeks at 2-8^o C or for at least six months at -20^o C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.

Applications :

Selected References :

Selected List of References Citing This Product

Neutralization 

To yield one-half maximal inhibition [ND ₅₀ ] of the biological activity of mIL-12 （2.0 ng/ml）, a concentration of 0.16-0.24 µg/ml of this antibody is required.

Elisa 

In a sandwich ELISA （assuming 100µl/well）, a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant murine IL-12 when used with 's biotinylated antigen affinity purified anti-murine IL-12 （500-P155GBt） as the detection antibody at a concentration of approximay 0.5-1.0 µg/ml.

Western Blot 

To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Country of Origin :

USA