黄曲霉素总量ELISA试剂盒(检饲料/粮食类，灵敏度0.04ppb,一步法)
一、原理

本试剂盒采用间接竞争ELISA方法，在微孔条上预包被偶联抗原，样本中的残留物黄曲素将和微孔条上预包被的偶联抗原竞争抗黄曲毒素抗体，加入酶标记物后，用TMB底物显色，样本吸光值与其所含残留物黄曲霉素的含量成负相关，与标准曲线比较即可得出相应残留物黄曲霉素的含量。

二、试剂盒特性
u  试剂盒灵敏度：   0.04ppb
u  孵育温度：       25℃
u  孵育时间：       30min～15min
u  样本检测下限
饲  料·················································2 ppb
花  生················································ 2 ppb
大  米················································ 1.6 ppb
玉  米················································  2 ppb
u  交叉反应率
黄曲霉素B1 ················································  100%
黄曲霉素B2 ················································  223%
黄曲霉素G1················································  78.4%
黄曲霉素G2 ················································  125%
u  样本回收率
饲料、大米、玉米、花生 ··················· 100±30%

三、试剂盒组成
1 微量测试孔 每条8孔，一板12条
2 标准液×6瓶
（1ml/瓶） 0ppb 0.04ppb
  0.12ppb 0.36ppb
  1.08ppb 3.24ppb
3 酶标记物 7ml 红色帽
4 抗体工作液 7ml 蓝色帽
5 底物A液 7ml 白色帽
6 底物B液 7ml 黑色帽
7 终止液 7ml 黄色帽
8 20X浓缩洗涤液 40ml 白色帽
9 2X浓缩复溶液 50ml 透明帽

四、样本前处理及实验分析步骤
   详见产品说明书。

五、储藏条件和保质期
1、 储藏条件：试剂盒于2～8℃保存，不要冷冻。
2、 保 质 期：该产品有效期为1年，生产日期见包装盒。